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pSIN-TRE-H2BeGFP-rtTA2

Plasmid

#165494

Purpose

Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

Depositor

Hector G. Palmer

Article

Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

-

Promoter

TetO-CMV

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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h3.7SP-Crtta

Plasmid

#20180

Depositor

Jeffrey Whitsett

Article

Tichelaar et al J Biol Chem. 2000 Apr 21. 275(16):11858-

Insert

h3.7SP-Crtta (SFTPC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2010

Availability

Academic Institutions and Nonprofits only
FUW-M2rtTA

Plasmid

#20342

Purpose

Lentiviral plasmid expressing the reverse tetracycline transactivator

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

M2rtTA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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PB-CA-rtTA Adv

Plasmid

#20910

Purpose

piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

rtTA-ON-Adv

Use

piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

tetracyclin-transactivator (rtTA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

Plasmid

#178528

Purpose

Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

rtTA, SspB-mScarlet-I-MYPT (1-169aa)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG, Tet responsive element

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV rtTA3 Hygro (w785-1)

Plasmid

#26730

Purpose

3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro

Depositor

Eric Campeau

Insert

Tetracycline repressor A3 mutant

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pVH235-Tier1-PmPGK1-rtTA

Plasmid

#169578

Purpose

Tier-1 vector encoding PmPGK1-driven rtTA expression (PmPGK1-rtTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline-controlled transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

Plasmid

#176027

Purpose

construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

rtTA3
Luc2
mtagBFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TRE-EGFP-EF1a-rtTA

Plasmid

#104454

Purpose

PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

Depositor

Volker Busskamp

Article

Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

Inserts

enhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

Plasmid

#229545

Purpose

Lentiviral construct to express rtTA and SKM proteins

Depositor

Domitilla Del Vecchio

Article

Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

Insert

rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hEF1a

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

Plasmid

#229797

Purpose

Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

rtTA-IRES-nls-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pHR hEF1A-BFP-P2A-rtTA-WPRE

Plasmid

#223288

Purpose

A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

BFP-P2A-rtTA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-TRE-CMV-Cre-rtTA

Plasmid

#165457

Purpose

Tetracycline inducible CRE expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

CRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tight TRE promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Tags

Expression

Mutation

homology arms for knock-in into first intron

Promoter

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

Plasmid

#85799

Purpose

donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Tags

Expression

Mutation

homology arms for knock-in into first intron

Promoter

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

Plasmid

#85800

Purpose

donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Tags

Expression

Mutation

homology arms for knock-in into first intron

Promoter

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge