-
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (700pM)
Plasmid#216714PurposeExpresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD1101 Integration Test Circuit Vector
Plasmid#139251PurposeIntegration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.DepositorInsertTus
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorUseTagsExpressionMammalianMutationPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
MG15-luc (mut p53 binding sites)
Plasmid#16443DepositorInsertp53-binding site (mutant) (TP53 )
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
MG15-gal (mut p53 binding sites)
Plasmid#16447DepositorInsertp53-binding site (mutant) (TP53 )
UseBeta-gal reporterTagsbeta-galExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hMALAT1 deletion 11 PARP1 binding sites
Plasmid#159327PurposeMammalian cell expression of human MALAT1 with deleted PARP1 binding sites. Contains CopGFP marker.DepositorInsertMALAT1 (MALAT1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion 11 PARP1 binding sitesPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites
Plasmid#120407Purposeenables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContains complete variable region with mutations …PromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP (TE)
Plasmid#61530PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TE mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)
Plasmid#120301PurposeAAV vector for expression of AcrIIC3 (no miR binding sites, control vector)DepositorInsertAcrIIC3
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)
Plasmid#216717PurposeExpresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteriaDepositorInsertHaloTag-RGD-PILATeS
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)
Plasmid#216715PurposeExpresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-RGD-PILATeS
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-2xmiR-1 target sites
Plasmid#120299PurposeAAV vector for expression of AcrIIA4 with two miR-1 binding sitesDepositorInsertAcrIIA4-2xmiR-1 binding sites
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)
Plasmid#120297PurposeAAV vector for expression of AcrIIA4 (no miR binding sites, control vector)DepositorInsertAcrIIA4
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)
Plasmid#120300PurposeAAV vector for expression of AcrIIC1 (no miR binding sites, control vector)DepositorInsertAcrIIC1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)
Plasmid#62579PurposeTranslational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)
Plasmid#48307DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)
Plasmid#48283DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic
Plasmid#32737DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic
Plasmid#32736DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only