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Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTarKO
Plasmid#110540Purposeone-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureusDepositorTypeEmpty backboneUseSynthetic Biology; Targeting vector for locus of …TagsExpressionMutationPromoterAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtAwoH
Plasmid#65017PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Fab1 (S. aureus)
Plasmid#61691PurposeInducible expression of the FabI enzyme from S. aureusDepositorInsertFabI
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ACP (from S. aureus)
Plasmid#63686PurposeInducible expression of Acyl Carrier Protein (ACP) from S. aureusDepositorInsertACP
UseTagsHisExpressionBacterialMutationcodon optimizedPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB38-NWMN2930
Plasmid#84457PurposepJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30DepositorInsertStaphylococcus aureus NWMN genomic region NWMN29-30
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA
Plasmid#64983PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2
Plasmid#64984PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
S. aureus DHPS
Plasmid#64079PurposeExpression of Staphylococcus aureus dihydropteroate synthaseDepositorInsertdihydropteroate synthase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2woH
Plasmid#65018PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-B1-B2-HIS-ybbr
Plasmid#117979PurposeSdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3-B1-B2
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-B1-HIS-ybbr
Plasmid#117980PurposeSdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3-B1
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B1-DK
Plasmid#117981PurposeSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B1
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B2-DK
Plasmid#117982PurposeSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrD_B1-DK
Plasmid#117983PurposeSdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7
Plasmid#64986PurposeExpresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5
Plasmid#64985PurposeExpresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7woH
Plasmid#65020PurposeExpresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only