Addgene

We narrowed to 79 results for: shigella

Showing: 41 - 60 of 79 results

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pNHA9

Plasmid

#223642

Purpose

pUC18 plasmid with rpsM promoter

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRMCD77

Plasmid

#173032

Purpose

Modified pET17b (Novagen) vector

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD

Plasmid

#183666

Purpose

N-terminally tagged Shigella flexneri IpgD for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b-LOV-ipaA L514K L531E

Plasmid

#40253

Depositor

Brian Kuhlman

Article

Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

Insert

LOV-ipaA L514K L531E

Use

Tags

6xHis

Expression

Bacterial

Mutation

L514K; L531E; Jα helix region hybridized (AsLOV2 …

Promoter

T7

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA_AT

Plasmid

#196243

Purpose

Backbone plasmid for cloning sgRNA

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pNHA5

Plasmid

#223647

Purpose

TurboID protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Insert

TurboID

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA6

Plasmid

#223648

Purpose

BioID protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Insert

BioID

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA10

Plasmid

#223554

Purpose

TurboID expression

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Insert

TurboID

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRMCD28

Plasmid

#167556

Purpose

Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

Type

Empty backbone

Use

Tags

PhoA'

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD70

Plasmid

#167557

Purpose

Plasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mapping

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

Type

Empty backbone

Use

Tags

LacZ'

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pNHA7

Plasmid

#223649

Purpose

birA protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Insert

BioID_G118R

Use

Tags

6His

Expression

Bacterial

Mutation

G118R

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA11

Plasmid

#223643

Purpose

Expression of ipgC-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Inserts

ipgC (ipgC )
turboID

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNHA12

Plasmid

#223644

Purpose

Expression of turboID-ipgC fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Inserts

ipgC (ipgC )
turboID

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNHA13

Plasmid

#223645

Purpose

Expression of mxiE-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Inserts

mxiE (mxiE )
turboID

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNHA14

Plasmid

#223646

Purpose

Expression of turboID-mxiE fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere 0:e00553-24

Inserts

mxiE (mxiE )
turboID

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EGFP-IpgD(C439S)

Plasmid

#183667

Purpose

N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

EGFP

Expression

Mammalian

Mutation

C439S

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD(C439S)

Plasmid

#183673

Purpose

Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

Expression

Yeast

Mutation

C439S

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA-R-gfp

Plasmid

#220927

Purpose

Expresses gfp using J23119 promoter and pgRNA_AT backbone

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

superfolder GFP

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCACTUS

Plasmid

#167559

Purpose

Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.

Depositor

Renato Morona

Article

Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do

Type

Empty backbone

Use

Suicide cloning vector, temperature sensitive.

Tags

Expression

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge