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pTXB1-APEX2-F

Plasmid

#160083

Purpose

Bacterial expression of C-terminally FLAG-tagged APEX2 peroxidase

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

APEX2 peroxidase

Use

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2m

Plasmid

#176842

Purpose

Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2

Plasmid

#111698

Purpose

Theophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Use

Tags

V5

Expression

Bacterial

Mutation

Promoter

hsp60-ribo

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Cyto-APEX2

Plasmid

#215552

Purpose

Stable, inducible expression in cytoplasm

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Endo-APEX2

Plasmid

#215553

Purpose

Stable, inducible expression in endosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

EGFP-2xFYVE-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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GALT-APEX2

Plasmid

#215554

Purpose

Stable, inducible expression in Golgi

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

APEX2-NES-GalT-EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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LAMTOR1-APEX2

Plasmid

#215556

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMTOR1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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5HT2A-APEX2

Plasmid

#215558

Purpose

Stable, inducible expression of 5HT2A

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)

Use

Human homologous recombination donor

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human PGK promoter

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD-APEX2

Plasmid

#154939

Purpose

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 APEX2-NES

Plasmid

#49386

Purpose

Expresses APEX2 in the cytosol of mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Flag - APEX2 - NES

Use

Tags

Flag

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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V5-GFP-APEX2-NES_pLX208

Plasmid

#202018

Purpose

expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-GFP-APEX2-NES

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL3

Plasmid

#149518

Purpose

Express APEX2-Galectin-3 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS3 (LGALS3 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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APEX2-V5-G3BP1_pTRE-G418

Plasmid

#202013

Purpose

expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

APEX2-V5-G3BP1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-Tight

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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3xflag-APEX2-LRRK2

Plasmid

#164622

Purpose

Expresses LRRK2 tagged with APEX2 and 3xFLAG

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

LRRK2 (LRRK2 Human)

Use

Tags

3xFLAG and APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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APEX2 - Actin in pEGFP

Plasmid

#66172

Purpose

APEX2 fused to the N-terminus of human beta actin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - human beta Actin (ACTB Synthetic, Human)

Use

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-COX4-dAPEX2

Plasmid

#117176

Purpose

Plasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL9

Plasmid

#149520

Purpose

Express APEX2-Galectin-9 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS9 (LGALS9 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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