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Plasmid#164845PurposeMammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
UseTags8his tag and HA leader sequenceExpressionMammalianMutationCodon-optimized for human cell expression. Has N5…PromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40
Plasmid#180758PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)DepositorInsertα-COPI-WD40
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Leu181Lys, Leu…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hACE2-LgBiT
Plasmid#215837PurposeExpresses hACE2 receptor with LgBiT tag in mammalian cellsDepositorInserthACE2-LgBiT (ACE2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Arg57Ala
Plasmid#180759PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)DepositorInsertα-COPI-WD40-Arg57Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Arg57Ala, Leu1…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Tyr139Ala
Plasmid#180761PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)DepositorInsertα-COPI-WD40-Tyr139Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Tyr139Ala, Leu…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Asp115Ala
Plasmid#180760PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)DepositorInsertα-COPI-WD40-Asp115Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Asp115Ala, Leu…PromoterCMVAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
Plasmid#208173PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
UseTagsStrep-tagExpressionMammalianMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala
Plasmid#208174PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationLys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
BA.2.86
Plasmid#208583PurposeMammalian expression vector for expressing BA.2.86 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncationDepositorInsertSARS-CoV-2 variant BA.2.86 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
DV.7.1
Plasmid#208584PurposeMammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncationDepositorInsertSARS-CoV-2 variant DV.7.1 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
XBB.1.5+R403K
Plasmid#208586PurposeMammalian expression vector for expressing XBB.1.5+R403K SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncationDepositorInsertSARS-CoV-2 variant XBB.1.5+R403K spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
XBB.1.5+F456L
Plasmid#208585PurposeMammalian expression vector for expressing XBB.1.5+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncationDepositorInsertSARS-CoV-2 variant XBB.1.5+F456L spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
XBB.1.5+K478R
Plasmid#208587PurposeMammalian expression vector for expressing XBB.1.5+K478R SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncationDepositorInsertSARS-CoV-2 variant XBB.1.5+K478R spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
XBB.1.5+L455F+F456L
Plasmid#208588PurposeMammalian expression vector for expressing XBB.1.5+L455F+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncationDepositorInsertSARS-CoV-2 variant XBB.1.5+L455F+F456L spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-1-RBD-8h
Plasmid#161821PurposeMammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T320-D518)PromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD
Plasmid#159999PurposeExpresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertSpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD Delta
Plasmid#214723PurposeExpresses SARS-CoV-2 Delta RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertReceptor-Binding Domain (S SARS-CoV-2)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD Omicron BQ.1.1
Plasmid#214725PurposeExpresses SARS-CoV-2 Omicron BQ.1.1 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertReceptor-Binding Domain (S SARS-CoV-2)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD Omicron XBB.1.5
Plasmid#214724PurposeExpresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertReceptor-Binding Domain (S SARS-CoV-2)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only