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Addgene
Showing: 41 - 60 of 71 results

  1. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid
    #164845
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Use
    Tags
    8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression. Has N5…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    164845-plasmid-map-sequence-id-322928

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  7. BA.2.86

    Plasmid
    #208583
    Purpose
    Mammalian expression vector for expressing BA.2.86 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant BA.2.86 spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208583-plasmid-map-sequence-id-409580

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  8. DV.7.1

    Plasmid
    #208584
    Purpose
    Mammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant DV.7.1 spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208584-plasmid-map-sequence-id-409583

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  9. XBB.1.5+F456L

    Plasmid
    #208585
    Purpose
    Mammalian expression vector for expressing XBB.1.5+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+F456L spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208585-plasmid-map-sequence-id-409584

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  10. XBB.1.5+R403K

    Plasmid
    #208586
    Purpose
    Mammalian expression vector for expressing XBB.1.5+R403K SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+R403K spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208586-plasmid-map-sequence-id-409585

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  11. XBB.1.5+K478R

    Plasmid
    #208587
    Purpose
    Mammalian expression vector for expressing XBB.1.5+K478R SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+K478R spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208587-plasmid-map-sequence-id-409578

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  12. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

    Plasmid
    #208172
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    Use
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208172-plasmid-map-sequence-id-432648

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  13. SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

    Plasmid
    #208173
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
    Use
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208173-plasmid-map-sequence-id-436198

    Enlarge

  14. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

    Plasmid
    #208174
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    Use
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208174-plasmid-map-sequence-id-436200

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  15. XBB.1.5+L455F+F456L

    Plasmid
    #208588
    Purpose
    Mammalian expression vector for expressing XBB.1.5+L455F+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+L455F+F456L spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208588-plasmid-map-sequence-id-409581

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  16. pcDNA3-SARS-1-RBD-8h

    Plasmid
    #161821
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T320-D518)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    161821-plasmid-map-sequence-id-316749

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Showing: 41 - 60 of 71 results