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Plasmid#111983PurposeSubcloning vector position 2DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only
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pACY.HRF_T7_term_HR1
Plasmid#111981PurposeSub cloning vector position 1DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNO41
Plasmid#101067PurposeBphP1-PpsR2 chromophore plasmid; ho1DepositorInsertho1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR78
Plasmid#90210PurposeExpresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyADepositorInsertsCcaS
Cph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT87
Plasmid#109302PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tagDepositorTypeEmpty backboneUseTagsOmpA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT90
Plasmid#109304PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tagDepositorTypeEmpty backboneUseTagsTamA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-GroEL/ES
Plasmid#83922PurposeCo-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.DepositorInsertsSkp
Gro EL/ES
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-TF
Plasmid#83921PurposeCo-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.DepositorInsertsSkp
Trigger Factor
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACYC_HA-ZmSAE1_ZmSAE2-FLAG
Plasmid#91944PurposeBacterial expression of maize SAE1 and SAE2DepositorInsertsHA-ZmSAE1
ZmSAE2-FLAG
UseTagsFLAG and HAExpressionBacterialMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC_HA-ZmSAE1
Plasmid#91942PurposeBacterial expression of maize SAE1DepositorInsertHA-ZmSAE1
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC_ZmSAE2-FLAG
Plasmid#91943PurposeBacterial expression of maize SAE2DepositorInsertZmSAE2-FLAG
UseTagsFLAGExpressionBacterialMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAK22
Plasmid#101741Purposeencoding two copies of T. thermophilus SecG for expression of SecYEG complexDepositorInsertThermus thermophilus HB8 SecG x 2
UseTagsNONEExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
UseTags6xHis tag and HRV3C cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG
Plasmid#91945PurposeBacterial expression of maize SAE1 and truncated SAE2DepositorInsertsHA-ZmSAE1
ZmSAE2-FLAG
UseTagsFLAG and HAExpressionBacterialMutationTruncation of ZmSAE2 to mimic a splice variantPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL0426 (CRISPR with spc3)
Plasmid#130642PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.DepositorInsertCRISPR(spacer-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0915 (CRISPR with target 3)
Plasmid#130643PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertCRISPR(target-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only