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AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159957

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159960

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159959

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159963

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-3xnls-Tq-Ca-FLITS

Plasmid

#145030

Purpose

piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Use

Tags

3xnls-Tq-Ca-FLITS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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hCXCR4-mTQ2

Plasmid

#110196

Purpose

Encodes for human CXCR4 coding sequence tagged with the mTQ2 FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCXCR4 (CXCR4 Human)

Use

Tags

mTurquoise (mTQ2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-mCherry

Plasmid

#191466

Purpose

Compare the intensity of mCherry to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mCherry

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-jRCaMP1b

Plasmid

#191468

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

jRCaMP1b

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-RCaMP1h

Plasmid

#191467

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

RCaMP1h

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-K-GECO1

Plasmid

#191469

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

K-GECO1

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2

Plasmid

#69624

Purpose

A FRET sensor for Galphai2 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2

Use

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Mutation

Promoter

CMV and CMV (IRES)

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

Plasmid

#69623

Purpose

A FRET sensor for Galphai1 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai-mTurquoise2

Use

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Mutation

Promoter

CMV and CMV (IRES)

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

Plasmid

#69625

Purpose

A FRET sensor for Galphai3 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2

Use

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Mutation

Promoter

CMV and CMV (IRES)

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

Plasmid

#145027

Purpose

Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Use

Lentiviral

Tags

3xnls-Tq-Ca-FLITS and H2B-Maroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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