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pSB1K3 containing mRFP1

Plasmid

#118082

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, mRFP1

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE_hygro_mRFP1_NRF2(C506S, L4A)

Plasmid

#136580

Purpose

mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (C506S, Leuzip mut) (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Mutation

The C506S mutation (C513S in the actual sequence …

Promoter

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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AEB-No-aptamer-controlmRFP1

Plasmid

#63848

Purpose

This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

No-aptamer control mRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

J23100

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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J23114-mRFP1-331Bb

Plasmid

#78279

Purpose

mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23114-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mRFP1

Plasmid

#104025

Purpose

Plasmid encoding 2ndVal mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mRFP1

Use

Low copy

Tags

Expression

Bacterial

Mutation

mRFP1 with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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J23110-mRFP1-331Bb

Plasmid

#78277

Purpose

mRFP1 behind constitutive promoter J23110 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23110-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEN_TT MEK5-DD-mRFP1

Plasmid

#47547

Purpose

Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MEK5-DD (MAP2K5 Human)

Use

Entry vector

Tags

mRFP

Expression

Mutation

S311D, T315D

Promoter

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
J23112-mRFP1-331Bb

Plasmid

#78278

Purpose

mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23112-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23103-mRFP1-331Bb

Plasmid

#78273

Purpose

mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23103-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23105-mRFP1-331Bb

Plasmid

#78275

Purpose

mRFP1 behind constitutive promoter J23105 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23105-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23101-mRFP1-331Bb

Plasmid

#78272

Purpose

mRFP1 behind constitutive promoter J23101 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23101-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Magenta

Plasmid

#183140

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Magenta

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Magenta

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Yellow

Plasmid

#183141

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Yellow

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Yellow

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Orange

Plasmid

#183143

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Orange

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Orange

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Violet

Plasmid

#183139

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Violet

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Violet

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Pink

Plasmid

#183142

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Pink

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Pink

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

W4 (modified T7A1)

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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