Addgene

Showing: 41 - 60 of 102 results

Results per page:

Sort results by:

pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE, modified BFP

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK293 (mAID-mCherry2-Hygro)

Plasmid

#72831

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Hygro

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

Plasmid

#67982

Purpose

Cas9 activity reporter with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK294 (mAID-mCherry2-Bsr)

Plasmid

#72832

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Bsr

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182862

Purpose

Hetero-tetramer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEYFP-mCherry2-mEGFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK377 (Hygro-P2A-mCherry2)

Plasmid

#121188

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mCherry2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry2-CD86-mEYFP(Q69K)

Plasmid

#190749

Purpose

Expression of CD86 double tagged with mCherry2 (N-term) and mEYFP(Q69K) (C-term) in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mCherry2 and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF820_U6-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138316

Purpose

Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT3_mCherry2-NLS-PEST_Puro

Plasmid

#213771

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT3_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT4_short_mCherry2-NLS-PEST_Puro

Plasmid

#213774

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT4_mCherry2-NLS-PEST_Puro

Plasmid

#213773

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT3_short_mCherry2-NLS-PEST_Puro

Plasmid

#213772

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT3_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT2_mCherry2-NLS-PEST_Puro

Plasmid

#213769

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT2_short_mCherry2-NLS-PEST_Puro

Plasmid

#213770

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT1_mCherry2-NLS-PEST_Puro

Plasmid

#213767

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-HKGT1_short_mCherry2-NLS-PEST_Puro

Plasmid

#213768

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EFS_mCherry2-NLS-PEST_Puro

Plasmid

#213775

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

EFS_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-mCherry2-mEGFP

Plasmid

#182859

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge