Addgene

We narrowed to 222 results for: hedgehog

Showing: 41 - 60 of 222 results

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PD13

Plasmid

#164530

Purpose

Expresses HA-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

HA and Ub noK

Expression

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pACSV-Ptc

Plasmid

#37675

Depositor

Philip Beachy

Article

Yao et al Cell. 2006 Apr 21;125(2):343-57.

Insert

ptc (ptc Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
GP735

Plasmid

#164537

Purpose

Expresses SmoM2-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened W539L (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

W539L

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GP736

Plasmid

#164532

Purpose

Express Smo-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP799

Plasmid

#164544

Purpose

Expresses Smo-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

WT

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBS cPtc2 (CT#4)

Plasmid

#13942

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 2001 Nov 1. 239(1):15-29.

Insert

Ptc2 (PTCH2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
GP940

Plasmid

#164539

Purpose

Expresses SmoPi-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

R455A

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP938

Plasmid

#164538

Purpose

Expresses SmoPi-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

R455A

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PD19

Plasmid

#164528

Purpose

Expresses Flag-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

FLAG and Ub noK

Expression

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX mPtch-Cterm

Plasmid

#58704

Purpose

used for anti-mouse Patched antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1238-1413)

Promoter

tac

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMal mPtch-Cterm

Plasmid

#58705

Purpose

used for anti-mouse Patched antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1205-1434)

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
M7 pdnPKA-GFP

Plasmid

#16716

Depositor

Randall Moon

Article

Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

Insert

dnPKA (Prkar1a Mouse)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Dominant negative. Mutations at cAMP binding site…

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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GP777

Plasmid

#164533

Purpose

Expresses Smo noK-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2-ptc-lu

Plasmid

#37678

Depositor

Philip Beachy

Article

Chen et al Cell. 1999 Aug 6;98(3):305-16.

Insert

ptc promoter (−758 to +130)

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

ptc

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
GP821

Plasmid

#164552

Purpose

Expresses Smo-FLAG K434R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GP758

Plasmid

#164541

Purpose

Expresses Sstr3-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Somatostatin receptor type 3 (Sstr3 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GP823

Plasmid

#164554

Purpose

Expresses Smo-FLAG K448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP822

Plasmid

#164553

Purpose

Expresses Smo-FLAG K444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP806

Plasmid

#164534

Purpose

Expresses Smo noK-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge