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Plasmid#219707PurposeLevel 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pSPCH-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I84 - GG0-pAHP6-GG1
Plasmid#219706PurposeLevel 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pAHP6-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTALE64G
Plasmid#35188PurposeGolden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP additionDepositorInsertTAL Effector-VP64-T2A-eGFP
UseTALENTagsExpressionMammalianMutation+63 C Terminus, VP64 Domain, T2A-GFPPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALE64
Plasmid#35189PurposeGolden Gate Compatible TALE Transcription Factor using VP64 DomainDepositorInsertTranscription Activator Like Effector Transcription Factor
UseTALENTagsExpressionMammalianMutation+63 C Terminus, VP64 DomainPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOGG010
Plasmid#113984PurposeVector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertRK2 (IncP)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG012
Plasmid#113986PurposeVector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectorsDepositorInsertPartition genes (parABCDE)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPARA2
Plasmid#200417PurposeGolden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TALEN_C63
Plasmid#40788PurposeGolden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsExpressionMammalianMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPARA1
Plasmid#200416PurposeGolden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50627PurposepRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN_C18
Plasmid#47974PurposeGolden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsExpressionMammalianMutationShortened C-Terminus,Codon Optimized FokI Endonuc…PromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NoTag-GG
Plasmid#215810PurposeCloning of target gene with no tagsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-HIS-Sangamo
Plasmid#40786Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsN Terminal 6xHISExpressionBacterialMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Sangamo-HIS
Plasmid#40787Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsC Terminal 6xHISExpressionBacterialMutation+63 C Terminus,Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NH10-GG
Plasmid#215806PurposeCloning of target gene with N terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-CH10-GG
Plasmid#215807PurposeCloning of target gene with C terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-MBP-TEV-GG
Plasmid#215794PurposeCloning of target gene with N terminal MBP tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-SUMO1-GG
Plasmid#215799PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-NHSTIIT-GG
Plasmid#215789PurposeCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits