-
Plasmid#206055Purposeexpression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functionalDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationL702PPromoterCMV/B-actinAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS W391A pcDNA3
Plasmid#206077PurposeCav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pCAGGS
Plasmid#206096Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Cav2.2 DomI and DomII
Plasmid#89891PurposeMammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2DepositorInsertCacna1b (CACNA1B )
UseTagsExpressionMammalianMutationStop codon after amino acid 1154 (this is a trunc…Promoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 EA2, R57A, R59A pMT2
Plasmid#206122Purposeexpression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currentsDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationCav2.1-P1217fs, R57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 DomI + DomII pMT2
Plasmid#206095Purposeexpression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tagDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]
Plasmid#26569DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
UseTags6XHis and V5ExpressionMammalianMutationDeletion of exons a10, 18a, and 24a. Contains …PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]
Plasmid#26567DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
UseTags6XHis and V5ExpressionMammalianMutationContains exons a10, 31a, 37b, and 46. Deletion…PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]
Plasmid#26571DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
UseTags6XHis and V5ExpressionMammalianMutationDeletion of exons a10, 18a, and 31a Contains e…PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]
Plasmid#26568DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
UseTags6XHis and V5ExpressionMammalianMutationcontains exons a10, 18a, 31a, 37b, and 46. Del…PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]
Plasmid#26570DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
UseTags6XHis and V5ExpressionMammalianMutationDeletion of exons a10, 18a, and 24a. Contains …PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS
Plasmid#206115Purposeexpression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320A, E670A, E1411A, E1707APromoterCMV/B-actinAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3
Plasmid#206121Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320A, E670A, E1411A, E1707APromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS
Plasmid#206098Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMV/B-actinAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3
Plasmid#206093PurposeCav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3
Plasmid#198451PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vectorDepositorInsertCav2.2 (CACNA1B Human)
UseTagsN-terminal GFP, internal double HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3
Plasmid#198914PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vectorDepositorInsertCav2.2 (CACNA1B Human)
UseTagsN-terminal GFP, internal double HAExpressionMammalianMutationexon 18a deleted, exon 19 starts 3 nucleotides ea…PromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human HAgHA-plus exon 18a - Short-Cterm - Arginine -in pcDNA3
Plasmid#198915PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vectorDepositorInsertCav2.2 (CACNA1B Human)
UseTagsN-terminal GFP, internal double HAExpressionMammalianMutationsnp rs2278973 ArgininePromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human -HAgHA-minus exon 18a -Short-Cterm - Arg -in pcDNA3
Plasmid#198916PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vectorDepositorInsertCav2.2 (CACNA1B Human)
UseTagsN-terminal GFP, internal double HAExpressionMammalianMutationexon 18a deleted, exon 19 starts 3 nucleotides ea…PromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTCAV-FLEx(loxP)-FlpO
Plasmid#67829PurposeCre-dependent expression of FlpO from CAV2 virusDepositorInsertFlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only