Skip to main content
Addgene
Showing: 41 - 51 of 51 results

  1. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

    Enlarge

  2. pTOX6

    Plasmid
    #127451
    Purpose
    Encodes Tse2 toxin and amilCP chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    tse2 toxin
    amilCP chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    pRha and tac
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127451-plasmid-map-sequence-id-251155

    Enlarge

  3. pTOX4

    Plasmid
    #127449
    Purpose
    Encodes YhaV toxin and amilCP chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    yhaV toxin
    amilCP chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    pRha and tac
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127449-plasmid-map-sequence-id-251117

    Enlarge

  4. pTOX5

    Plasmid
    #127450
    Purpose
    Encodes MqsR toxin and amilCP chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    mqsR toxin
    amilCP chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    pRha and tac
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127450-plasmid-map-sequence-id-249680

    Enlarge

  6. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

    Enlarge

  7. pUS250

    Plasmid
    #198322
    Purpose
    Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cumate-inducible
    Available since
    April 12, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    198322-plasmid-map-sequence-id-390064

    Enlarge

  10. pGA-blue

    Plasmid
    #196336
    Purpose
    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196336-plasmid-map-sequence-id-393157

    Enlarge

  11. mUAV

    Plasmid
    #102680
    Purpose
    Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts
    Depositor
    Naomi Nakayama
    Article
    Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi
    Insert
    J23106:B0034:amilCP:rrnBT1-T7TE
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    102680-plasmid-map-sequence-id-200980

    Enlarge

Showing: 41 - 51 of 51 results