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Showing: 41 - 60 of 121 results

  1. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Article
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Expression
    Mutation
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    129531-plasmid-map-sequence-id-255632

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  10. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid
    #115137
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA2.1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115137-plasmid-map-sequence-id-220492

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  11. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

    Plasmid
    #115136
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115136-plasmid-map-sequence-id-220499

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  12. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

    Plasmid
    #115139
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA3.1 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115139-plasmid-map-sequence-id-220497

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  13. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid
    #115140
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA3 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115140-plasmid-map-sequence-id-220495

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  14. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid
    #115138
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized AcrVA2 anti-CRISPR protein
    Use
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115138-plasmid-map-sequence-id-220494

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  20. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid
    #120294
    Purpose
    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)
    Depositor
    Dominik Niopek
    Article
    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545
    Insert
    C-terminal fragment of SpyCas9
    Use
    AAV and CRISPR
    Tags
    split-intein
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    120294-plasmid-map-sequence-id-232735

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Showing: 41 - 60 of 121 results