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pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

Plasmid

#148521

Purpose

Mammalian Expression of HseIF4G1-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1-LIVVtoAAAA (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation M432V (natural variant is…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EBP3-YLLVtoAAAA_Z

Plasmid

#148149

Purpose

Mammalian Expression of Hs4EBP3-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EBP3-YLLVtoAAAA (EIF4EBP3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4EBP1_50-83-YLLVtoAAAA_AA

Plasmid

#148244

Purpose

Mammalian Expression of HseIF4EBP1_50-83-YLLVtoAAAA

Depositor

Elisa Izaurralde

Insert

HseIF4EBP1_50-83-YLLVtoAAAA (EIF4EBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EBP1-YLLVtoAAAA_Z

Plasmid

#148146

Purpose

Mammalian Expression of Hs4EBP1-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EBP1-YLLVtoAAAA (EIF4EBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPD389 VPR-ZF43(AAAA)

Plasmid

#138826

Purpose

Constitutive expression of VPR-ZF43(AAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(AAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A, R71A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD388 VP64-ZF43(AAAA)

Plasmid

#138822

Purpose

Constitutive expression of VP64-ZF43(AAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43(AAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A, R71A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD113 VP16-ZF43(AAAA)

Plasmid

#138818

Purpose

Constitutive expression of VP16-ZF43(AAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(AAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A, R71A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-LIR-mutant (LIRAAAA)

Plasmid

#187981

Purpose

Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

DDDW335-338AAAA (LIR mutant), silent mutation siR…

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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GFP KNL1 wt hardened and RVSF->AAAA

Plasmid

#45225

Depositor

Michael Lampson, Iain Cheeseman

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Use

Tags

Expression

Mutation

RVSF mutated to AAAA

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
mCherry KNL1 wt hardened and RVSF->AAAA

Plasmid

#45226

Depositor

Michael Lampson, Iain Cheeseman

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

mCherry KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Use

Tags

TEV S15

Expression

Mutation

RVSF 58 AAAA

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
Mouse GFP-PRMT7 LDIG(69-72)AAAA

Plasmid

#65044

Purpose

Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

Insert

PRMT7 (Prmt7 Mouse)

Use

Bacterial propagation

Tags

GFP

Expression

Mammalian

Mutation

L69A D70A I71A G72A

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

Plasmid

#196829

Purpose

Express dpdpCas13b with AAAA mutation and Clover

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

dpspCas13b(AAAA)

Use

Tags

3XFLAG and NES

Expression

Mammalian

Mutation

K367A, D369A and K370A

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

Plasmid

#201965

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E198A, R199A,E200A, D201A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-SceIF4E_25-213-KKKKtoAAAA_AF

Plasmid

#148729

Purpose

Bacterial Expression of SceIF4E_25-213-KKKKtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4E_25-213-KKKKtoAAAA (CDC33 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP1_50-83-YLLVtoAAAA-GB1_AA

Plasmid

#148278

Purpose

Bacterial Expression of Hs4EBP1_50-83-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP1_50-83-YLLVtoAAAA (EIF4EBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGex-CS-DmCup_296-425-YLLLtoAAAA-GB1_Z

Plasmid

#148171

Purpose

Bacterial Expression of DmCup_296-425-YLLLtoAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_296-425-YLLLtoAAAA (cup Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-QHTRtoAAAA_AC

Plasmid

#148421

Purpose

Mammalian Expression of HsRck_296-472-QHTRtoAAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-QHTRtoAAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI

Plasmid

#148950

Purpose

Mammalian Expression of HsGIGYF2_1-718-YFWFtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2_1-718-YFWFtoAAAA (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AE

Plasmid

#148580

Purpose

Mammalian Expression of HsGIGYF2_1-180-YYMLtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-YYMLtoAAAA (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

Plasmid

#148523

Purpose

Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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