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Plasmid#220475Purposeheterologous expression of PA1472 under endogenous speG E. coli promoterDepositorInsertPA1472
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1377
Plasmid#220476Purposeheterologous expression of PA1377 under endogenous speG E. coli promoterDepositorInsertPA1377
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fg vector
Plasmid#213464PurposeDestination vector for cloning and transformation into TSI locus 1.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTorD-H6
Plasmid#169039PurposeEscherichia coli TorD with C-terminal 6xHis in pET28a.DepositorInsertTorD-H6
UseTags6xHisExpressionBacterialMutationThe mutation N at position 46 was changed back to…PromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_MTOR
Plasmid#183311PurposeAll-in-One CRISPRko system with a guide RNA that targets MTOR geneDepositorInsertMTOR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA
Plasmid#178102PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA
Plasmid#178103PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA
Plasmid#178108PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA
Plasmid#178109PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA
Plasmid#173209PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DEPTOR_DEP
Plasmid#109882PurposeProtein expression and purification of DEPTOR_DEPDepositorInsertDEPTOR_DEP (DEPTOR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_OTOR-2/2
Plasmid#91382PurposeProtein expression and purification of human SH3 domain construct OTOR-2/2DepositorInsertOTOR-2/2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_OTOR-1/2
Plasmid#91328PurposeProtein expression and purification of human SH3 domain construct OTOR-1/2DepositorInsertOTOR-1/2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO1_2
Plasmid#158011PurposeExpression vector for the de novo designed protein Expression_vector_RO1_2DepositorInsertde novo designed protein RO1_2
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO1_5
Plasmid#158012PurposeExpression vector for the de novo designed protein Expression_vector_RO1_5DepositorInsertde novo designed protein RO1_5
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO1_8
Plasmid#158013PurposeExpression vector for the de novo designed protein Expression_vector_RO1_8DepositorInsertde novo designed protein RO1_8
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO1_9
Plasmid#158014PurposeExpression vector for the de novo designed protein Expression_vector_RO1_9DepositorInsertde novo designed protein RO1_9
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_1
Plasmid#158015PurposeExpression vector for the de novo designed protein Expression_vector_RO2_1DepositorInsertde novo designed protein RO2_1
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_5
Plasmid#158016PurposeExpression vector for the de novo designed protein Expression_vector_RO2_5DepositorInsertde novo designed protein RO2_5
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_6
Plasmid#158017PurposeExpression vector for the de novo designed protein Expression_vector_RO2_6DepositorInsertde novo designed protein RO2_6
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only