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Gal:mCherry-uTEV3

Plasmid

#135453

Purpose

Yeast expression of uTEV3 fused to mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-uTEv3

Use

Tags

mCherry

Expression

Yeast

Mutation

S219V mutation improves stability , I138T/S153N/T…

Promoter

Gal

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-10xHis-Super TEV

Plasmid

#193833

Purpose

Expresses high-efficiency TEV protease in bacterial cells

Depositor

Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

Article

Lau et al protocols.io, doi: 10.17504/protocols.io

Insert

TEV protease

Use

Tags

6xHis

Expression

Bacterial

Mutation

T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

Plasmid

#169192

Purpose

To express SARS-CoV-2 Papain-like protease in E. coli

Depositor

John Diffley

Article

Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

Insert

6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)

Use

Unspecified

Tags

6His-TEV

Expression

Mutation

Codon optimised for E. coli

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Jim Wells, Nirao Shah

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV5

Inserts

Caspase 3 (CASP3 Human, Synthetic)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Tags

Expression

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Use

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-TO-nTEVPhcZhccTEVP

Plasmid

#116066

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

nTEVPhcZhccTEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-nTEVPtvZtvcTEVP

Plasmid

#116067

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

nTEVPtvZtvcTEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-FKBP-nTEVP

Plasmid

#179675

Purpose

ER-associated FKBP split nTEVP (Rapalog inducible)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

FKBP-nTEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-NTEVp(WT)

Plasmid

#137831

Purpose

Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-NTEVp(WT)

Use

Tags

3x FLAG and NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-CTEVp(WT)

Plasmid

#137832

Purpose

Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CTEVp(WT)

Use

Tags

3x FLAG and NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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V2-MESA-35F-Tev

Plasmid

#84503

Purpose

MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-Tev

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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V1-MESA-45F-Tev

Plasmid

#84501

Purpose

MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V1-MESA-45F-Tev

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Ca-LiPPI-CLAPon1.0 (TEVp)

Plasmid

#213409

Purpose

Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

CaM-V5-TEVp

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Bacteroidetes-bGSDM-TEV

Plasmid

#222101

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Bacteroidetes-bGSDM-TEV ORF

Use

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

TEV protease site replaces residues E242–G248

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Use

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-puro-2A-TEVp

Plasmid

#99610

Purpose

Lentivirus expressing puromycin resistance gene and TEV protease

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Puromycin resistance (PAC gene)
TEV protease

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-FRB-cTEVP

Plasmid

#179676

Purpose

ER-associated FRB split-cTEVP (Rapalog inducible)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

FRB (MTOR Human, Synthetic)

Use

Tags

cTEVP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (TEVp)

Plasmid

#213407

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

HA-arrestin-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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