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Plasmid#166002PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(C164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(L166)
Plasmid#165995PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(L166)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)
Plasmid#181792PurposeExpresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
XE160 XDshHA-pt mutant PDZ-CS2+
Plasmid#16780DepositorInsertDishevelled (dvl2.L Frog)
UseXenopus expressionTagsHAExpressionMutationpoint mutation in the binding pocket of the PDZ b…PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE161 XDshHA-triple pt mutant-CS2+
Plasmid#16779DepositorInsertDishevelled (dvl2.L Frog)
UseXenopus expressionTagsHAExpressionMutationtriple point mutation outside of the PDZ binding …PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)
Plasmid#181790PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)
Plasmid#181791PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)
Plasmid#22131DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralTagsExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targeti…PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)
Plasmid#22132DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralTagsExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targeti…PromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH
Plasmid#179586PurposeePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)DepositorInsertNLS-NG-T2A-hSHH
UsePiggybacTagsNeonGreenExpressionMutationPromoterTREAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZE12 sonic hedgehog CMV
Plasmid#17061DepositorInsertsonic hedgehog (shha Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:axFgf8-pPH:vsvged
Plasmid#111159PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertaxFgf8
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationwtPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-TT-PA-CRE
Plasmid#179584PurposeePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)DepositorInsertPA-MAG-CRE
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
L13-Arl13bGFP
Plasmid#40879Purpose3rd generation transfer vectorDepositorInsertArl13b (Arl13b Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-Arl13b12
Plasmid#40872DepositorInsertArl13b (Arl13b Mouse)
UseTagsGFPExpressionMammalianMutationno stop codonPromoterCMVAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-Arl13b11
Plasmid#40873DepositorInsertArl13b (Arl13b Mouse)
UseTagsHis and V5ExpressionMammalianMutationno stop codonPromoterCMVAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-Arl13b89
Plasmid#40874DepositorInsertArl13b (Arl13b Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Arl13b2
Plasmid#40871DepositorInsertArl13b (Arl13b Mouse)
UseGateway entryTagsExpressionMutationno stop codonPromoterAvailable sinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Arl13b1
Plasmid#40870DepositorInsertArl13b (Arl13b Mouse)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:
Plasmid#179585PurposeePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)DepositorInsertNLS-NG-T2A-hSHH
UsePiggybacTagsNLS-NG-T2AExpressionMutationPromoterCAGpAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only