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pHH0103_SH3D19-1/5

Plasmid

#91339

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-1/5 (SH3D19 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-2/4

Plasmid

#91316

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-2/4 (SH3PXD2B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-1/4

Plasmid

#91317

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-1/4 (SH3PXD2B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D21-2/3

Plasmid

#91297

Purpose

Protein expression and purification of human SH3 domain construct SH3D21-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D21-2/3 (SH3D21 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3D21-3/3

Plasmid

#91298

Purpose

Protein expression and purification of human SH3 domain construct SH3D21-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D21-3/3 (SH3D21 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3TC2-1/2

Plasmid

#91294

Purpose

Protein expression and purification of human SH3 domain construct SH3TC2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3TC2-1/2 (SH3TC2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3PXD2B-4/4

Plasmid

#91257

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-4/4 (SH3PXD2B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3D19-2/5

Plasmid

#91272

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-2/5 (SH3D19 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3KBP1-3/3

Plasmid

#91250

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-3/3 (SH3KBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AcGFP-MSH3

Plasmid

#215122

Purpose

Expresses AcGFP-MSH3 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MSH3 (MSH3 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A WT

Plasmid

#69813

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SH3BP4

Plasmid

#46891

Purpose

expresses HA tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Fyn-SH3_5WT_S25C

Plasmid

#118824

Purpose

Bacterial expression of the SH3 domain of human Fyn

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

FYN (FYN Human)

Use

Tags

6-His-TEV tag

Expression

Bacterial

Mutation

S25C; See depositor comments below.

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1

Plasmid

#136647

Purpose

Expression of SHBP1-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (SH3BP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-SH3(2)-5R

Plasmid

#112089

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-5R (NCK1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

construct contains only repeats of the second SH3…

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Rlucrarr3-Flash3

Plasmid

#74131

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Use

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Promoter

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SH3PXD2A-PX (1-126)

Plasmid

#119115

Purpose

Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SH3PXD2A-PX (1-126) (SH3PXD2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge