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pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Use

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLX208-beta-arrestin2-HA-TEV219

Plasmid

#104846

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-βarrestin2-LOV-Turbo_pCDNA3

Plasmid

#199671

Purpose

expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Tags

Myc and βarrestin2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pIDS-RESTwt full-length

Plasmid

#109161

Purpose

Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Use

Tags

Expression

Insect

Mutation

Promoter

p10

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-beta-arrestin2-HA-TEV219

Plasmid

#104845

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Co-Rest/RCOR1 [K72/8R]

Plasmid

#140066

Purpose

Mammalian Expression Plasmid of anti-Co-Rest/RCOR1 (Human). Derived from hybridoma K72/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Co-Rest/RCOR1 (Human) recombinant mouse monoclonal antibody (RCOR1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 K11,12R GFP

Plasmid

#35412

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K11,12R

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIDS-RESTmut full-length

Plasmid

#109162

Purpose

Encodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Use

Tags

Expression

Insect

Mutation

Cys402Ser

Promoter

p10

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-G4DBD-CREST (C91)

Plasmid

#22963

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Use

Tags

G4DBD

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
REST corepressor 2_L (OZ553)

Plasmid

#28078

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
REST corepressor 2_R (OZ554)

Plasmid

#28079

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
Anti-Co-Rest/RCOR1 [K72/2R]

Plasmid

#222145

Purpose

Mammalian Expression Plasmid of anti-Co-Rest/RCOR1 (Human). Derived from hybridoma K72/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Co-Rest/RCOR1 (Homo sapiens) recombinant mouse monoclonal antibody. (RCOR1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

Plasmid

#194680

Purpose

CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Hpep1-betaArrestin2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CMV sTurboID (C)-Arrestin-GFP

Plasmid

#153010

Purpose

Expresses split-TurboID C-terminal fragment fused to arrestin

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C)(G74)-Arrestin

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-bArrestin2-TevC-P2A-TdTomato

Plasmid

#89869

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

R173H in TdTomato (please see depositor comments …

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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APL0030 SFFV-bArrestin2-MCP (FLP-IN)

Plasmid

#233436

Purpose

SFFV driven ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ024 CMV-TO-bArrestin2-MCP (FLP-IN)

Plasmid

#233379

Purpose

ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Crestin-pro (JDW 1320)

Plasmid

#229830

Purpose

A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Crestin promoter, c-fos minimal promoter, and b-globin intron

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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