Addgene

We narrowed to 201 results for: Mycobacterium

Showing: 41 - 60 of 201 results

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pGFPHYG2

Plasmid

#30173

Depositor

Lalita Ramakrishnan

Article

Cosma et al Infect Immun. 2006 Jun . 74(6):3125-33.

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

GFP

Expression

Mutation

GFPmut3 allele has improved brightness.

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pTEC19

Plasmid

#30178

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Crimson

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pYUB1062-GFP

Plasmid

#117695

Purpose

Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

Depositor

Elizabeth Fullam

Article

Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

Type

Empty backbone

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pYS1

Plasmid

#117488

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Article

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

Che9c 60–61, sacB, aph

Use

Deletion generation

Tags

Expression

Mutation

Promoter

Acetamide

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3WT

Plasmid

#225738

Purpose

To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria

Depositor

Nicole Sampson

Insert

Myc-igr3WT

Use

Tags

c-Myc

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-M1

Plasmid

#225734

Purpose

To express M1 (13-193) in E. coli

Depositor

Nicole Sampson

Insert

mce3R

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 13-193

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-Rv2416c_wild

Plasmid

#37649

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Rv2416c (eis Mycobacterium tuberculosis)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pET28c-Ku

Plasmid

#161936

Purpose

Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

Depositor

Harris Wang

Article

Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

Insert

ku (mku Mycobacterium tuberculosis)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7P

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBUN82

Plasmid

#60675

Purpose

overexpression of M. smegmatis alrA gene in Mycobacteria

Depositor

Raul Barletta

Article

Caceres et al J Bacteriol. 1997 Aug;179(16):5046-55.

Insert

alrA

Use

E.coli-mycobacterium shuttle vector

Tags

Expression

Mutation

Promoter

hsp60

Available since

Dec. 23, 2014

Availability

Academic Institutions and Nonprofits only
pUV15-pHGFP

Plasmid

#70045

Purpose

encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

Depositor

Sabine Ehrt

Article

Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

Insert

phGFP

Use

Mycobacterium tuberculosis expression vector

Tags

Expression

Mutation

Promoter

mycobacterial promoter Psmyc

Available since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSBY1_FnCpf1cg

Plasmid

#104622

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSBY3_ligD

Plasmid

#104624

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

FnCpf1 codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSBY2_ligD(FnCpf1)

Plasmid

#104623

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

crRNA to ligD

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Pj23119

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMV306G13+FFluc

Plasmid

#26157

Depositor

Siouxsie Wiles, Brian Robertson

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Firefly luciferase

Use

Mycobacteria integrating vector

Tags

Expression

Mutation

Codon optimized for Mycobacterium tuberculosis. …

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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AiP909-Bxb1-AttP-PGK-gb2-Neo-PGK-Bxb1-AttP-SA-hygro2

Plasmid

#181778

Purpose

Bxb1 AttP in TIGRE landing pad vector

Depositor

Tanya Daigle

Insert

Neomycin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLAM12

Plasmid

#26908

Depositor

Graham Hatfull

Article

van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

Insert

Acetamidase Promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only
pNHEJ-recX-sacB

Plasmid

#158718

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pmyc

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pYC1640

Plasmid

#158719

Purpose

CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNHEJ-recAmu-sacB

Plasmid

#158717

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recA

Use

Tags

Expression

Bacterial

Mutation

R61C

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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