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Plasmid#30173DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsGFPExpressionMutationGFPmut3 allele has improved brightness.PromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC19
Plasmid#30178DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsCrimsonExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYUB1062-GFP
Plasmid#117695PurposeExpress recombinant proteins with GFP-His fusion in Mycobacterium smegmatisDepositorTypeEmpty backboneUseTagsGFP and HisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYS1
Plasmid#117488PurposeGeneration of unmarked gene deletions in Mycobacterium smegmatisDepositorInsertChe9c 60–61, sacB, aph
UseDeletion generationTagsExpressionMutationPromoterAcetamideAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMV261-igr3WT
Plasmid#225738PurposeTo express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in MycobacteriaDepositorInsertMyc-igr3WT
UseTagsc-MycExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-M1
Plasmid#225734PurposeTo express M1 (13-193) in E. coliDepositorInsertmce3R
UseTags6xHisExpressionBacterialMutationaa 13-193PromoterAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Rv2416c_wild
Plasmid#37649DepositorInsertRv2416c (eis Mycobacterium tuberculosis)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28c-Ku
Plasmid#161936PurposeExpresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purificationDepositorInsertku (mku Mycobacterium tuberculosis)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a_Msm_RbpA
Plasmid#87928PurposeMycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposesDepositorInsertRbpA
UseTagsExpressionBacterialMutationPromoterT7PAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBUN82
Plasmid#60675Purposeoverexpression of M. smegmatis alrA gene in MycobacteriaDepositorInsertalrA
UseE.coli-mycobacterium shuttle vectorTagsExpressionMutationPromoterhsp60Available sinceDec. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUV15-pHGFP
Plasmid#70045Purposeencodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cellsDepositorInsertphGFP
UseMycobacterium tuberculosis expression vectorTagsExpressionMutationPromotermycobacterial promoter PsmycAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBY3_ligD
Plasmid#104624PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligDDepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationFnCpf1 codon-optimized C.glutamicumPromoterhsp60Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBY1_FnCpf1cg
Plasmid#104622PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1DepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationcodon-optimized C.glutamicumPromoterhsp60Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBY2_ligD(FnCpf1)
Plasmid#104623PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligDDepositorInsertcrRNA to ligD
UseCRISPRTagsExpressionBacterialMutationPromoterPj23119Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+FFluc
Plasmid#26157DepositorInsertG13 promoter + Firefly luciferase
UseMycobacteria integrating vectorTagsExpressionMutationCodon optimized for Mycobacterium tuberculosis. …PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
AiP909-Bxb1-AttP-PGK-gb2-Neo-PGK-Bxb1-AttP-SA-hygro2
Plasmid#181778PurposeBxb1 AttP in TIGRE landing pad vectorDepositorInsertNeomycin
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLAM12
Plasmid#26908DepositorInsertAcetamidase Promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recX-sacB
Plasmid#158718PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.DepositorInsertrecX
UseTagsExpressionBacterialMutationPromoterPmycAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC1640
Plasmid#158719PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC2085
Plasmid#158721PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only