Addgene

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pAGM18012

Plasmid

#104657

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

N terminal GST-Tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAGT861

Plasmid

#104658

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

N terminal HIS-Tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG41722

Plasmid

#104659

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

EL#1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG41744

Plasmid

#104660

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

EL#2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG41766

Plasmid

#104661

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

EL#3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-41308

Plasmid

#104641

Purpose

Level 0 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-1287

Plasmid

#104642

Purpose

Level 0 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-P2

Plasmid

#104644

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-P3

Plasmid

#104645

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-TP1

Plasmid

#104649

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-124

Plasmid

#104635

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4

Use

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS-TDP-43

Plasmid

#162617

Purpose

Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-2A-oG-WPRE

Plasmid

#102984

Purpose

Lentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Enhanced Green Fluorescent Protein (eGFP)
Optimised Rabies glycoprotein (oG)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP-43

Plasmid

#162614

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

1-155-I152L

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huBim_Ex3

Plasmid

#85532

Purpose

Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Bim

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mKate2

Plasmid

#162624

Purpose

Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mKate2

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

Plasmid

#102986

Purpose

AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

membrane-targeted SNAP protein after FLP-mediated recombination
Nuclear-targeted eGFP after FLP-mediated recombination

Use

AAV; Adeno associated viral vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pQUAST-syb-spCFP1-10

Plasmid

#73699

Purpose

Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spCFP1–10 chimera

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V

Plasmid

#162615

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

M337V

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge