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pLITMUS-rpoN-cI-J23106-geneIII

Plasmid

#80843

Purpose

Phagemid (encodes M13 gene III and lambda cIopt).

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
Lambda cI

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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5xtetO (tet3) Gene Desert

Plasmid

#131339

Purpose

gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

Desert-gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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CJ19 (miR-1006 minigene)

Plasmid

#17760

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1006 minigene

Use

Drosophila metallothionein gene promoter drives e…

Tags

Expression

Insect

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pUC19 gene 60 delta gap

Plasmid

#49091

Purpose

Template for generating gene 60 mRNA, coding gap deletion

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and coding gap STOP have been deleted.

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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CJ20 (miR-1003 minigene)

Plasmid

#17761

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene

Use

Drosophila metallothionein gene promoter drives e…

Tags

Expression

Insect

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pGL0_27 [Tfd gene VIII]

Plasmid

#198940

Purpose

Level 0 part

Depositor

Charlie Gilbert, Cultivarium Tools, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Tfd gene VIII

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 thru gap

Plasmid

#49160

Purpose

Template for generating gene 60 mRNA truncated two codons after coding gap

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

gene 60 mRNA truncated two codons after coding ga…

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 to gap + Cys

Plasmid

#49159

Purpose

Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 600

Use

Tags

Expression

Mutation

Coding gap and downstream sequence have been dele…

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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CJ30 (miR-1003 minigene)

Plasmid

#17767

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene

Use

Drosophila p-element vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
CJ31 (miR-1006 minigene)

Plasmid

#17768

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1006 minigene

Use

Drosophila p-element vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO - RBM28 E5 GT>A minigene

Plasmid

#169270

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 GT>A minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 GT>A minigene (RBM28 Human)

Use

Tags

Expression

Mammalian

Mutation

NM_018077.2:c.(541+1_541+2delinsA)

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 WT minigene

Plasmid

#169271

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 WT minigene (RBM28 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 G>T minigene

Plasmid

#169272

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 G>T minigene (RBM28 Human)

Use

Tags

Expression

Mammalian

Mutation

NM_018077.2:c.(946G>T)

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. pyogenes scaffold CARGO array

Plasmid

#191316

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMS-dsRed2-WT LMNA-GFP minigene

Plasmid

#128230

Purpose

Lamin A splicing reporter

Depositor

Tom Misteli

Article

Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

Insert

lamin A (LMNA Human)

Use

Tags

GFP and SV40-driven dsRed2 to identify transfecte…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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nontarget S. pyogenes scaffold CARGO array

Plasmid

#191319

Purpose

Contains an array of nontargeting guide RNAs

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-PRM-B0034-geneVI

Plasmid

#80858

Purpose

Accessory plasmid (encodes M13 gene VI)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Insert

M13 gene VI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lambda Prm

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PrpoN-Cro-PBBa_J23106-geneIII

Plasmid

#134353

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Cro

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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Gene Trap - 24XMS2 Lentiviral Vector

Plasmid

#174198

Purpose

Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

Depositor

Daniel Larson

Article

Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

Insert

iRFP

Use

Lentiviral

Tags

24X MS2 hairpins, Blasticidin, and T2A

Expression

Mutation

Promoter

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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