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Plasmid#107577PurposeB. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromotergapdh promoter - B. megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107578PurposeB. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic BiologyTagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfbp promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBGN155
Plasmid#74992PurposeGFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plantsDepositorTypeEmpty backboneUseReporter plasmidTagsGFP (1–155)/GN155ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGC155-MXMT
Plasmid#79818PurposeGFP(S65T)-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsGC155/GFP (155–239)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGN155-MXMT
Plasmid#79817PurposeGFP(S65T)-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsGN155/GFP (1–155)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWKS2
Plasmid#139410PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.DepositorInsertGFP S65T
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
UseTagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …PromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean ME
Plasmid#103971PurposePlasmid encoding mCerulean MEDepositorInsertmCerulean ME
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
7TGC
Plasmid#24304DepositorInsert7xTcf-eGFP // SV40-mCherry
UseLentiviralTagsExpressionMutationEGFP is GFP with S65T and F64L mutationsPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
7TGP
Plasmid#24305DepositorInsert7xTcf-eGFP // SV40-PuroR
UseLentiviralTagsExpressionMutationEGFP is GFP with S65T and F64L mutationsPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
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7TG
Plasmid#24314DepositorInsert7xTcf-eGFP
UseLentiviralTagsExpressionMutationEGFP is GFP with S65T and F64L mutationsPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKT0210
Plasmid#8724DepositorInsertyECFP A206R
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0174
Plasmid#8720DepositorInsertyECFP
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N144I, M153T, V163APromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0101
Plasmid#8721DepositorInsertyECFP
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163APromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0232
Plasmid#8727DepositorInsertyECFP
UseTags13x Myc and Codon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163APromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0212
Plasmid#8725DepositorInsertyECFP A206R
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0221
Plasmid#8726DepositorInsertyECFP
UseTags3HA and Codon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163APromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT222
Plasmid#40942DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only