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Plasmid#172562PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Streptomycin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pELlR4
Plasmid#172545PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Carbenicillin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1
Plasmid#145369PurposeGateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tagDepositorTypeEmpty backboneUseTagsHis and eGFPExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP3A4
Plasmid#67104PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP3A4 (CYP3A4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2D6
Plasmid#67090PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2D6 (CYP2D6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCCL-CellCycle
Plasmid#132429PurposeExpressing Fucci cell cycle indicator in mammalian cellsDepositorInserthCdt, hGeminin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
UseTagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO LV
Plasmid#202001PurposeExpresses HELLO neoantigen in tumor cellsDepositorInsertHELLO
UseLentiviralTagsFLAGExpressionMutationPromoterpGKAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
fwYellow chromoprotein
Plasmid#117841PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coliDepositorInsertpromoter, RBS, fwYellow
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFEC
Plasmid#67060PurposeStandard for cell-free expression benchmarking.DepositorInsertTFEC (TFEC Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDicAID_nCas9-PmCDA_NptII_Della
Plasmid#91694PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDellaDepositorInsertSpCas9
UseTagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRPV1exCellHalo
Plasmid#220307PurposeMammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helicesDepositorInsertrTRPV1exCellHalo (Trpv1 Rat)
UseTagscircularly permutated Halotag (Deo et al., Nat Ch…ExpressionMammalianMutationlinker included to insert cpHaloTag (PromoterCMV promoterAvailable sinceJuly 30, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-MSP1D1delltaH4
Plasmid#71713PurposeMembrane scaffold protein (MSP), helix 4 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorInsertIL6 (IL6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-mKelly2
Plasmid#119783PurposeExpresses mKelly2 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly2
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 JellyOp
Plasmid#41432DepositorInsertBox jellyfish opsin
UseTags1D4 epitope at 3' endExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
Salmonella MTAN
Plasmid#64041PurposeExpression of S. enterica MTAN in E. coliDepositorInsertMethylthioadenosine nucleosidase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCF12
Plasmid#67113PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF12 (TCF12 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only