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Plasmid#207394PurposeExpresses NlaCas7-p65 activator fusion in mammalian cellsDepositorInsertEF1a-NlaCas7-p65-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPV-C1-crRNA-cloning-EF1a-BA
Plasmid#204623PurposepiggyBac vector for crRNA cloning and expression. Blasticidin selectable.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat
Plasmid#178883PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.DepositorInsertU6-Nla repeat-BbsI-Nla repeat
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-mRxxFP1(AfeI)-iP-A
Plasmid#204625PurposeCloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-NlaCascade-NLS-6xHis
Plasmid#180213PurposeExpressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.ColiDepositorInsertNlaCas5-Cas8-Cas7
UseTags6XHis and NLSExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-EGxxFP(DMDex45_10)-iP-A
Plasmid#204621PurposeSingle-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A
Plasmid#204622PurposeSingle-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.DepositorInsertmRFP1
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-All-in-one-hCascade
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA
Plasmid#204620PurposeExpression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.DepositorInsertcrRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-457
Plasmid#200213PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 H25A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-458
Plasmid#200214PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 E184A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-459
Plasmid#200215PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 K214A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-460
Plasmid#200216PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-461
Plasmid#200217PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-462
Plasmid#200218PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E, R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EFS-1/1
Plasmid#91456PurposeProtein expression and purification of human SH3 domain construct EFS-1/1DepositorInsertEFS-1/1 (EFS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas45
Plasmid#82390PurposesgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg2(NC)-SH2
Plasmid#46472DepositorInsertPLCg2(NC) (PLCG2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 524-749PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only