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Plasmid#122186PurposeBacterial genome editing plasmid with spectinomycin resistance markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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pMS12: pDonor(AvCAST)
Plasmid#168145PurposeAvCAST donor harboring non-functional cargo.DepositorInsertsAvCAST right end (RE)
AvCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS13: pTarget(AvCAST)
Plasmid#168146PurposeTarget plasmid containing AvPSP1 and attachement site for AvCAST.DepositorInsertsPAM for AvCAST + AvPSP1
glmS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS8: pTns(AvCAST)
Plasmid#168141PurposeInducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.DepositorInsertsAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS17: pTwinStrep-SUMO-AvTnsD
Plasmid#168150PurposeAvTnsD expression construct for protein purification.DepositorInsertAvCAST TnsD
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS15: pTwinStrep-SUMO-AvTnsB
Plasmid#168148PurposeAvTnsB expression construct for protein purification.DepositorInsertAvCAST TnsB
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS16: pTwinStrep-SUMO-AvTnsC
Plasmid#168149PurposeAvTnsC expression construct for protein purification.DepositorInsertAvCAST TnsC
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS14: pTwinStrep-SUMO-AvTnsA
Plasmid#168147PurposeAvTnsA expression construct for protein purification.DepositorInsertAvCAST TnsA
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL2960
Plasmid#70694PurposepDU1-E. coli shuttle vector (Km-r) with extended polylinkerDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS10: pTns(AvCAST)_ΔTnsD
Plasmid#168143PurposeInducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.DepositorInsertAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL2697
Plasmid#70693PurposepDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinkerDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD
Plasmid#168144PurposeInducible expression of AvCAST TnsA, TnsB and TnsC proteins.DepositorInsertAvCAST Tns proteins (TnsA, TnsB and TnsC)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS9: pTns(AvCAST)_ΔTniQ
Plasmid#168142PurposeInducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.DepositorInsertsAvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015
Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
UseTagsExpressionBacterialMutationGvpA T6A-I48VPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS7: pCascade(AvCAST)_entry
Plasmid#168140PurposeInducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.DepositorInsertsAvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015
Plasmid#202024PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
UseTagsExpressionBacterialMutationGvpA T6A-L40APromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
Plasmid#221080PurposeSEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)DepositorInsertACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS1: pHelper(AvCAST)_AvPSP1
Plasmid#168133PurposeInducible expression of AvCAST proteins with crRNA targeting AvPSP1.DepositorInsertsAvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS6: pHelper(AvCAST)_entry_ΔTnsD
Plasmid#168139PurposeInducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.DepositorInsertsAvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS5: pHelper(AvCAST)_entry_ΔTniQ
Plasmid#168138PurposeInducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.DepositorInsertsAvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only