These results may also be relevant.
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pcDNA3-InPAkt
Plasmid#181914PurposeIndicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertInPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFP
Plasmid#181979PurposeHuman RUNX1A gene overexpressionDepositorInsertRUNX1A (human) (RUNX1 Human)
UseLentiviralTagsHA (Human influenza hemagglutinin)ExpressionMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-AC8(1-106)
Plasmid#181958PurposeResidues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.DepositorInsertEGFP-AC8(1-106) (Adcy8 Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniE-DBCO
Plasmid#181985PurposeUsed to label proteins with the MiniR genetically encoded tagDepositorInsertMiniE-DBCO
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniR-DBCO
Plasmid#181984PurposeUsed to label proteins with the MiniE genetically encoded tagDepositorInsertMiniR-DBCO
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-miniE
Plasmid#181987PurposeMiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporterDepositorInsertTfR1-MiniE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10HA
Plasmid#181941PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xHA
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mCherry-p115(S240A)
Plasmid#181997PurposeMammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.DepositorInsertmCherry-p115(S240A) (ARHGEF1 Human)
UseTagsmCherryExpressionMammalianMutationSerine 240 changed to alanine in p115.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10FLAG
Plasmid#181940PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xFLAG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.Cas9.P2A.BSD
Plasmid#181981PurposeLentiviral Cas9 expressionDepositorInsertCas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD317.8
Plasmid#181951PurposeIPTG-inducible expression of NarXDepositorUseTagsExpressionBacterialMutationPromoterNarX-Ptac; lacI-PlacIqAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-blast-3Myc:HaloTag:3Myc
Plasmid#181943PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertHaloTag
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.HA.RUNX1C.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#181978PurposeHuman RUNX1C gene overexpressionDepositorInsertRUNX1C (human) (RUNX1 Human)
UseLentiviralTagsHA (Human influenza hemagglutinin)ExpressionMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16-mHP1alpha
Plasmid#181910PurposeBacterial protein expression vector for HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10Myc
Plasmid#181939PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xMyc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1: TfR1-CoilE
Plasmid#181988PurposeCoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporterDepositorInsertTfR1-CoilE
UseTagsCoilEExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-mHP1alpha
Plasmid#181911PurposeBacterial protein expression vector for HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorInsertAKAP79-AKAR4 (AKAP5 Human)
UseTagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-InPAkt
Plasmid#181915PurposeIndicator of Phosphoinositides using Akt; targeted to the plasma membrane.DepositorInsertpm-InPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationContains the following mutations with respect to …PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf_LamB1
Plasmid#181999PurposeCMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell linesDepositorInsertSTf-LamB1
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only
