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Showing: 101 - 150 of 406 results

  4. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid
    #65662
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    introduced HindIII site (final goal to exchange F…
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65662-plasmid-map-sequence-id-111633

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  7. pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

    Plasmid
    #65660
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed glutamic acid 97 to alanine, lysine 100 t…
    Promoter
    CMV, 2x Tet
    Available since
    June 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    65660-plasmid-map-sequence-id-111635

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  10. pFRT-TODestFLAGHA_RBPMS_KDR_111del

    Plasmid
    #65659
    Purpose
    FLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    introduced stop codon such as to produce deletion…
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65659-plasmid-map-sequence-id-111636

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  29. PET01-TAF5-ex8-Minigene-SRSF1 motif WT

    Plasmid
    #218972
    Purpose
    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RSV LTR
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218972-plasmid-map-sequence-id-436110

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  37. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209044-plasmid-map-sequence-id-413636

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  38. pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

    Plasmid
    #210820
    Purpose
    Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)
    Depositor
    Luke Chao
    Article
    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7
    Inserts
    Prominin 1 (PROM1 Human)
    Protocadherin 21 (CDHR1 Human)
    Use
    Tags
    Myc, 3xFlag and TwinStrep, 10xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and CMV / IRES2
    Available since
    July 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210820-plasmid-map-sequence-id-417732

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  49. pcDNA5-3xFLAG-TAF5-∆ex8

    Plasmid
    #209050
    Purpose
    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TAF5 deltaexon-8
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO inducible promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209050-plasmid-map-sequence-id-414801

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  50. pcDNA5-miniTurbo-TAF5-∆ex8

    Plasmid
    #209052
    Purpose
    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TAF5 deltaexon-8
    Use
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO inducible promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209052-plasmid-map-sequence-id-414803

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Showing: 101 - 150 of 406 results