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Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
UseTags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-mko1
Plasmid#78467PurposeBroad range bacterial fluorescence labelingDepositorInsertmKO1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_linker
Plasmid#211731PurposeLinker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regionsDepositorInsertLinker fragment SARS-CoV-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-R3R2
Plasmid#105109Purposecreate your own 2in1 constructsDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57 moxDendra2
Plasmid#89791Purposebacterial expression of moxDendra2DepositorInsertmoxDendra2
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-phzAG
Plasmid#141106PurposeContains a seven gene phzABCDEFG operon BioBrickDepositorArticleInsertphzABCDEFG
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-mKo1
Plasmid#78477PurposeBroad range bacterial fluorescence labelingDepositorInsertmKO1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
PUC57_BAG3wt-eGFP
Plasmid#98182PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorInsertBAG3wt-eGFP (BAG3 Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 APEX
Plasmid#40306PurposeA monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).DepositorInsertpea APEX (Synthetic)
UseUnspecifiedTagsExpressionMutationK14D, W41F, E112K (relative to wild-type)PromoterAvailable sinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC19-gRNA
Plasmid#137776PurposeContains the T7 promoter and gRNA scaffold and is used for the in vitro transcription of gRNAsDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionMutationpUC19 BsaI destroyedPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-Top-T
Plasmid#87309PurposeExpresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Top" transcript
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-dsRedExpress
Plasmid#78470PurposeBroad range bacterial fluorescence labelingDepositorInsertdsRedExpress
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-Bottom-T
Plasmid#87310PurposeExpresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Bottom" transcript
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-inmCherry
Plasmid#60239PurposeHIV-1 transfer vector that encodes mcherry reporter gene interrupted by intronDepositorInsertinmCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC57-4eCOL2A1
Plasmid#97211PurposeFor cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectorsDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseVector is designed for cloning and generation of …TagsExpressionMutationPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-R1R4
Plasmid#105110Purposecreate your own 2in1 constructsDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-inGFPt
Plasmid#60237PurposeHIV-1 transfer vector that encodes gfp-turbo reporter gene interrupted by intronDepositorInsertinGFPt
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC119 T7 hTRmin
Plasmid#127858PurposeT7 RNA Pol for in vitro transcriptionDepositorInserthTR (TERC Human)
UseTagsExpressionMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Pd-mChe
Plasmid#183172PurposeConstruct for transient expression of plasmodesmata-targeted mCherry in plants.DepositorInsertPd-mCherry
UseTagsAtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR29_00091361
Plasmid#121209PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR29_00091361
UseGolden gateTagsExpressionMutationMagnaporthe griseaPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-mPlum
Plasmid#78474PurposeBroad range bacterial fluorescence labelingDepositorInsertmPlum
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57kan-T7-gRNA-U6 V2
Plasmid#115520PurposeTo construct multiple sgRNA expression vector for CRISPRDepositorInsertsgRNA scaffolf-U6 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-mChe
Plasmid#183159PurposePlant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5vio-2300
Plasmid#170987PurposeEncodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.DepositorInsertSh-CAST sgRNA 2300 violacein
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterplacIAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/DdmEGFP
Plasmid#154304Purposecloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideumDepositorInsertmEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-Apple1
Plasmid#132749PurposeEncodes an iconic representation of an appleDepositorInsertApple1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AksgRNA
Plasmid#121955PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAkCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
UseTags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCdeltaSS-26X
Plasmid#108218PurposeYeast reporter gene with glucocorticoid response element (GRE) upstream of CYC1 promoter driving lacZ expressionDepositorInsertGRE-CYC1-lacZ
UseTagsExpressionYeastMutationPromoterCYC1Available sinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-iSpinach
Plasmid#186053Purposetemplate for co-transcriptional RNA folding assaysDepositorInsertT7A1-rrnG-iSpinach
UseTagsnoExpressionBacterialMutationPromoterT7A1Available sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
PUC57_BAG3P209L-eGFP
Plasmid#98183PurposeCodes for full length, GFP tagged mutant Bag3DepositorInsertBAG3P209L-eGFP (BAG3 Human)
UseTagsGFPExpressionBacterialMutationP209LPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA
Plasmid#121954PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18-H1 RNAi
Plasmid#87355PurposeRNAi expression vector based on H1 promoter and pUC18 vector backboneDepositorInsertH1 RNA Promoter from U87MG glioma cells
UseRNAi; Shrna expression vectorTagsExpressionMammalianMutationPromoterH1 RNAAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5-2300
Plasmid#171788PurposeEncodes sgRNA target 2300 and Gentamicin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-G2ZYP2
Plasmid#103110PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertG2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA3.8
Plasmid#121958PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA, short version
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6MBW6
Plasmid#103102PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-crRNA4n96
Plasmid#123640PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertcrRNA scaffold 4n96
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCdeltaSS-ERE
Plasmid#108217PurposeYeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expressionDepositorInsertERE-CYC1-lacZ
UseTagsExpressionYeastMutationPromoterCYC1Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19_Ntag_BbsI-Mammalian
Plasmid#186674PurposeN-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.DepositorInsertMammalian N-terminal Tag
UseTags3xFlag-3xHAExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q927P4
Plasmid#103131PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_FR13_APikL2
Plasmid#123648PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertFR13_APikL2
UseGolden gateTagsExpressionMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-eCFP
Plasmid#183155PurposePlant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFPDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
SCP1-pUC119
Plasmid#21846DepositorInsertSCP1 (super core promoter 1)-pUC119
UsePuc derivative - a cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PP7v3
Plasmid#107230PurposeExpresses 24x optimized PP7 stem loops (version 3) in bacterial cellsDepositorInsert24xPP7v3
UseTags3x FLAG-tag and P2A siteExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-8xHis
Plasmid#101699Purpose8xHis in domestication vectorDepositorInsertAATG-[8xHis]-AGCC
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-THG73tRNA
Plasmid#21292DepositorInsertT. thermophila tRNA Glutamine (CUA)-G73
UseT. thermophilaTagsExpressionMutationN/APromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only