Showing: 101 - 150 of 188 results
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Plasmid#92419PurposeFluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.DepositorInsertMCOLN1 (Mcoln1 Mouse)
UseTagseGFPExpressionMammalianMutationSynthetic gene, codon optimized for human.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EPG
Plasmid#117978PurposeExpress EPG in mamalian cellsDepositorInsertElectromagnetic perceptive gene
UseTagsHIS and v5ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-biotin-myc- mCE
Plasmid#82475PurposeExpresses mRNA capping enzyme containing N terminal biotin and myc tagDepositorInsertBiotin (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
UseTagsHis tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 p37
Plasmid#169015PurposeExpresses GST-p37 (human, codon optimized for expression in bacteria)DepositorInsertp37
UseTagsGSTExpressionBacterialMutationsequence was codon optimized for expression in E.…PromotertacAvailabilityAcademic Institutions and Nonprofits only -
pFL GST-UBXN2A
Plasmid#169016PurposeExpresses GST-UBXN2A (human) in insect cellsDepositorInsertUBXN2A
UseTagsGSTExpressionInsectMutationPromoterpolyhedrin promoterAvailabilityAcademic Institutions and Nonprofits only -
pET28P_dCrk
Plasmid#131145PurposeExpresses 6XHis::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTags6XHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse–insD
Plasmid#191228PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 in the protein seque…PromoterPH and p10AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*- MmH-MH4R
Plasmid#196409PurposeExpress sfGFP with TAG and enzymes for biosynthesizing DOPADepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV5-FLAG-Gene33
Plasmid#21633DepositorInsertGene33 (Errfi1 Rat)
UseTagsFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF-HA-TSAd-d239-74
Plasmid#84560Purposemammalian expression of TSAd-d239-74DepositorInsertTSAd (SH2D2A Human)
UseTagsHAExpressionMammalianMutationdeletion of aa 239-274PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-myc- mCE(Del 25C)
Plasmid#82472PurposeExpresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminalDepositorInsertmyc-mCE-C terminal deleted (Rngtt Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSW646 - pML1>>
Plasmid#115995Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSW648 - pREV>>
Plasmid#115996Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSW683 - pAHP6>>
Plasmid#115998Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSW725 - pUFO>>
Plasmid#116000Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL41 - pSMXL5>>
Plasmid#116007Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpSMXL5:GR-LhG4:tSMXL5:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL42 - pLTP1>>
Plasmid#116008Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpLTP1:GR-LhG4:tLT1:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL43 - pNST3>>
Plasmid#116009Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL44 - pVND7>>
Plasmid#116010Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL45 - pPXY>>
Plasmid#116011Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpPXY:GR-LhG4:tPXY:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL46 - pWOX4>>
Plasmid#116012Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL47 - pSCR>>
Plasmid#116013Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL48 - pAt2g38380>>
Plasmid#116014Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAT2G38380:GR-LhG4:tAt4g24550:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVL58 - pAPL>>
Plasmid#116015Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAPL:GR-LhG4:tAPL:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse+R111Q–insD
Plasmid#191229PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 and replaced arginin…PromoterPH and p10AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos TRAM Flag
Plasmid#41551DepositorInsertTRAM (TICAM2 Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/ TO myc-cCE-bio (cCE-bio)
Plasmid#82473PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive formDepositorInsertMyc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
UseTagsTEV-Biotin and mycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTIGER_tdTomato::3xFLAG::dCrk
Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and tdTomatoExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA1
Plasmid#131139PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorInsertdCrk gRNA1 (Crk Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA2
Plasmid#131140PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorInsertdCrk gRNA1 (Crk Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FCM101
Plasmid#194298PurposeE. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6DepositorInsertFCM101
UseTagsHis tag, FLAG tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOpen-K12polLF (exo-)
Plasmid#165522PurposeDNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.DepositorInsertDNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRII-Topo Lhx2
Plasmid#45873DepositorInsertLhx2 in situ probe (Lhx2 Mouse)
UseIn situTagsExpressionMutationcontains bp# 1809-2217 of NM_010710.3PromoterAvailabilityAcademic Institutions and Nonprofits only -
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pSLIRES-11 (CC#13)
Plasmid#15145DepositorTypeEmpty backboneUseAdapter vector for cloning into prisapTagsIRESExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pICSL4723-P1
Plasmid#191786PurposeLevel P Golden gate accepterDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS6-mPSD95a-TSa-HA
Plasmid#42235PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis ratesDepositorInsertPSD95
UseTagsTSa-HAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS6-mPSD95a-TSt-HA
Plasmid#42237PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis ratesDepositorInsertPSD95
UseTagsTSt-HAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCis GFPuv-SS Himar1 A7
Plasmid#84686PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCis mCherry-SS Himar1 A7
Plasmid#84687PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSAM_Cam
Plasmid#91570PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJJB296
Plasmid#107691Purpose35SPromoter-LwaCas13a-HSPTerminatorDepositorInsertCas13a
UseTagsExpressionPlantMutationPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
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pENTR-CrkII
Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 101 - 150 of 188 results