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Showing: 101 - 150 of 7088 results

  1. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104406-plasmid-map-sequence-id-204465

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  2. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Expression
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  3. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid
    #104412
    Purpose
    transient expression of dCas9-dDNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    dDNMT3a
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    C706S
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104412-plasmid-map-sequence-id-204224

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  4. p-dCas9-SET7-H297G-Hygro

    Plasmid
    #104415
    Purpose
    transient expression of dCas9-dSET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    dSET7
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H297G
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104415-plasmid-map-sequence-id-203185

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  5. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

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  6. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214121-plasmid-map-sequence-id-423619

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  7. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91854-plasmid-map-sequence-id-176529

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  10. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid
    #159907
    Purpose
    Mutagenesis of Netrin1
    Depositor
    Larry Zweifel
    Insert
    Netrin1 (Ntn1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159907-plasmid-map-sequence-id-317313

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  11. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid
    #159909
    Purpose
    Mutagenesis of Nts
    Depositor
    Larry Zweifel
    Insert
    Nts (Nts Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159909-plasmid-map-sequence-id-317315

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  12. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid
    #159903
    Purpose
    Mutagenesis of Oprk1
    Depositor
    Larry Zweifel
    Insert
    Oprk1 (Oprk1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159903-plasmid-map-sequence-id-317307

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  13. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid
    #159920
    Purpose
    Mutagenesis of Vamp2
    Depositor
    Larry Zweifel
    Insert
    Vamp2 (Vamp2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159920-plasmid-map-sequence-id-317338

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  14. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

    Plasmid
    #159919
    Purpose
    Mutagenesis of Vamp1
    Depositor
    Larry Zweifel
    Insert
    Vamp1 (Vamp1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159919-plasmid-map-sequence-id-317336

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  18. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  19. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

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  21. pX330-U6-MYC-Guide-hSpCas9

    Plasmid
    #228574
    Purpose
    Expression of human MYC gRNA; CRISPR mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    MYC gRNA (MYC Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228574-plasmid-map-sequence-id-457356

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  22. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  24. pAAV-CMV-FLEX-SaCas9-U6-sgTh

    Plasmid
    #159901
    Purpose
    Mutagenesis of Th
    Depositor
    Larry Zweifel
    Insert
    Th (Th Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159901-plasmid-map-sequence-id-317304

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  25. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

    Plasmid
    #159906
    Purpose
    Mutagenesis of Dcc
    Depositor
    Larry Zweifel
    Insert
    Dcc (Dcc Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159906-plasmid-map-sequence-id-317312

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  26. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR and Synthetic Biology; Pmagic gateway entr…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

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  28. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid
    #159916
    Purpose
    Mutagenesis of Penk
    Depositor
    Larry Zweifel
    Insert
    Penk (Penk Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159916-plasmid-map-sequence-id-317327

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  29. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

    Plasmid
    #159918
    Purpose
    Mutagenesis of Drd2
    Depositor
    Larry Zweifel
    Insert
    Drd2 (Drd2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159918-plasmid-map-sequence-id-317332

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  30. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

    Plasmid
    #159917
    Purpose
    Mutagenesis of Drd1
    Depositor
    Larry Zweifel
    Insert
    Drd1 (Drd1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159917-plasmid-map-sequence-id-317329

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  31. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid
    #159912
    Purpose
    Mutagenesis of Kcnd3
    Depositor
    Larry Zweifel
    Insert
    Kcnd3 (Kcnd3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159912-plasmid-map-sequence-id-317319

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  32. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid
    #159904
    Purpose
    Mutagenesis of Kcnn3
    Depositor
    Larry Zweifel
    Insert
    Kcnn3 (Kcnn3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159904-plasmid-map-sequence-id-317308

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  33. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid
    #159913
    Purpose
    Mutagenesis of Kcnj6
    Depositor
    Larry Zweifel
    Insert
    Kcnj6 (Kcnj6 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159913-plasmid-map-sequence-id-317320

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  34. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

    Plasmid
    #159911
    Purpose
    Mutagenesis of Kcnq3
    Depositor
    Larry Zweifel
    Insert
    Kcnq3 (Kcnq3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159911-plasmid-map-sequence-id-317318

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  35. pSEVA47_dCas9-GFP_NOT_iC

    Plasmid
    #231418
    Purpose
    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)
    Depositor
    Alfonso Jaramillo
    Article
    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev
    Inserts
    dCas9
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    231418-plasmid-map-sequence-id-468903

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  39. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid
    #159908
    Purpose
    Mutagenesis of Htr2a
    Depositor
    Larry Zweifel
    Insert
    Htr2a (Htr2a Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159908-plasmid-map-sequence-id-317314

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  40. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid
    #160587
    Purpose
    Nickase D10A Cas9 for Nt and Ct fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160587-plasmid-map-sequence-id-322079

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  41. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid
    #160588
    Purpose
    Nickase D10A Cas9 for Nt fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160588-plasmid-map-sequence-id-322078

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  42. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  44. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  47. p-dCas9-HDAC1-H141A-Hygro

    Plasmid
    #104417
    Purpose
    transient expression of dCas9-dHDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dHDAC1 (HDAC1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H141A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104417-plasmid-map-sequence-id-204223

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Showing: 101 - 150 of 7088 results