Skip to main content
Addgene
Showing: 101 - 150 of 543 results

  2. pOZ-FH-C Bach1 K52R

    Plasmid
    #73645
    Purpose
    Retroviral expression of Bach1 K52Rmutant (helicase null)
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Use
    Retroviral
    Tags
    FLAG and His
    Expression
    Mammalian
    Mutation
    K52R (helicase null)
    Promoter
    unknown
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. pCEP4-CH115gp160-QES.c14-754*

    Plasmid
    #123282
    Purpose
    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (CH115) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    123282-plasmid-map-sequence-id-235248

    Enlarge

  27. pCEP4-CH111-QES.c13.L544Y

    Plasmid
    #123267
    Purpose
    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (AD8) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations V208M, …
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123267-plasmid-map-sequence-id-235228

    Enlarge

  32. pOZ-FH-C Bach1 K141A/K142A

    Plasmid
    #73646
    Purpose
    Retroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Use
    Retroviral
    Tags
    FLAG and His
    Expression
    Mammalian
    Mutation
    K141A/K142A ( mlh1 binding mutant)
    Promoter
    unknown
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only

  33. pCDNA3.1 Bach1 K141A/K142A

    Plasmid
    #73642
    Purpose
    Mammalian expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Use
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    K141A/K142A ( mlh1 binding mutant)
    Promoter
    CMV
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  35. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    113865-plasmid-map-sequence-id-220242

    Enlarge

  36. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

    Enlarge

  42. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

    Enlarge

  44. CAG-MCS-WPRE-SV40 - GCH1

    Plasmid
    #230814
    Purpose
    AAV cloning vector for expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230814-plasmid-map-sequence-id-466629

    Enlarge

  45. CaMKII-MCS-WPRE-SV40 - GCH19

    Plasmid
    #230823
    Purpose
    AAV cloning vector for expression under the CaMKII promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230823-plasmid-map-sequence-id-457140

    Enlarge

  46. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid
    #230820
    Purpose
    AAV cloning vector for expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230820-plasmid-map-sequence-id-457137

    Enlarge

  47. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid
    #224787
    Purpose
    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    crBACH1-1, crBACH1-2, crBACH1-3,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and hU6-2xTetO
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224787-plasmid-map-sequence-id-450827

    Enlarge

  48. gWiz_BM40-vGLUT1-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218438
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vGLUT1 Fab domain
    Depositor
    Axel Brunger
    Insert
    vGLUT1-Vh-hIgG1-CH2
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218438-plasmid-map-sequence-id-434501

    Enlarge

  49. gWiz_BM40-vMAT1-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218440
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vMAT1 Fab domain
    Depositor
    Axel Brunger
    Insert
    vMAT1-Vh-hIgG1-CH3
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218440-plasmid-map-sequence-id-431092

    Enlarge

  50. gWiz_BM40-vMAT2-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218442
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vMAT2 Fab domain
    Depositor
    Axel Brunger
    Insert
    vMAT2-Vh-hIgG1-CH4
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218442-plasmid-map-sequence-id-431084

    Enlarge

Showing: 101 - 150 of 543 results