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Showing: 81 - 120 of 2625 results

  1. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid
    #171383
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized minidCas13X.1-REPAIRv2
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171383-plasmid-map-sequence-id-337350

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  2. CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR

    Plasmid
    #171382
    Purpose
    Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized dCas13X.1-REPAIRv2
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171382-plasmid-map-sequence-id-337349

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  4. pAAV.CMV.Luc.IRES.EGFP.SV40

    Plasmid
    #105533
    Purpose
    AAV expression of Luciferase and EGFP from CMV promoter
    Depositor
    James M. Wilson
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105533-plasmid-map-sequence-id-202367

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  5. pAAV.CMV.ffLuciferase.SV40

    Plasmid
    #105532
    Purpose
    AAV expression of firefly luciferase from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105532-plasmid-map-sequence-id-202366

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  6. pENN.AAV.CMVs.TurboRFP.WPRE.RBG

    Plasmid
    #105548
    Purpose
    AAV expression of TurboRFP from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1 and AAV8
    Insert
    TurboRFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105548-plasmid-map-sequence-id-202402

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  7. pAAV.CMV.LacZ.bGH

    Plasmid
    #105531
    Purpose
    AAV expression of LacZ from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV5 and AAV8
    Insert
    LacZ
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105531-plasmid-map-sequence-id-202365

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  11. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

    Plasmid
    #182380
    Purpose
    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters
    Depositor
    Chunghun Lim
    Article
    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1
    Inserts
    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Changed CDS sequence from 'ccacgttcaagtcacga…
    Promoter
    CMV promoter and SV40 promoter
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182380-plasmid-map-sequence-id-358102

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  12. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  13. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  18. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105530-plasmid-map-sequence-id-454420

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  19. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

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  20. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  26. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid
    #105537
    Purpose
    AAV expression of Cre from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105537-plasmid-map-sequence-id-202428

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  27. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  28. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  30. pAdx-CMV-copGFP

    Plasmid
    #73346
    Purpose
    Express copGFP under CMV promoter
    Depositor
    Kazuhiro Oka
    Insert
    CMV-copGFP
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73346-plasmid-map-sequence-id-137759

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  31. pAdx-CMV-tdTomato

    Plasmid
    #73347
    Purpose
    Express tdTomato under CMV promoter
    Depositor
    Kazuhiro Oka
    Insert
    CMV-tdTomato
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73347-plasmid-map-sequence-id-138092

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  33. pMCMV3

    Plasmid
    #85711
    Purpose
    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences
    Depositor
    Ulrich Koszinowski, Martin Messerle
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MIEPmouse CMV MIEP
    Available since
    April 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    85711-plasmid-map-sequence-id-177907

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  35. pAdx-CMV-LacZ

    Plasmid
    #73345
    Purpose
    Express LacZ under control of CMV promoter
    Depositor
    Kazuhiro Oka
    Insert
    CMV-LacZ
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73345-plasmid-map-sequence-id-138098

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Showing: 81 - 120 of 2625 results