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Plasmid#97012PurposeRosa26 targeting vector for intersectional Cre and FLP responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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CRISPR_TRAC
Plasmid#164993PurposeExpression of gRNA targeting TCRalpha constant locusDepositorInsertgRNA against TRAC
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_DMS3g24
Plasmid#89244PurposePlasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3DepositorInsertCRISPR
UseTagsExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nesprin3-C-18
Plasmid#58194PurposeLocalization: Nuclear Membrane , Excitation: N/A, Emission: N/ADepositorInsertNesprin3 (SYNE3 Human)
UseTagsSNAP tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
LentiCRISPRCreb5gRNA
Plasmid#195021PurposeSequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domainDepositorInsertCreb5 gRNA (CREB5 Bovine)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_1
Plasmid#164986PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_2
Plasmid#164987PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA
Plasmid#97010PurposeRosa26 targeting vector for FLP-responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR2
Plasmid#167001PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 2 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR1
Plasmid#167000PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 1 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_369
Plasmid#111823PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 349-369
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_AMBRA1#2
Plasmid#174153PurposeLentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 geneDepositorInsertAMBRA (AMBRA1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_TRBC
Plasmid#164994PurposeExpression of gRNA targeting TCRbeta constant locusDepositorInsertgRNA against TRBC
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#113252PurposepUC19 backbone with E. coli CRISPR arrayDepositorInsertE. coli CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_314
Plasmid#111822PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 294-314
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
epiCRISPRn
Plasmid#135961PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAH01SpR
Plasmid#128159PurposepCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDSDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR3
Plasmid#167002PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 3 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_AMBRA1#1
Plasmid#174152PurposeLentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 geneDepositorInsertAMBRA (AMBRA1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC_CRISPR_Cat
Plasmid#120368PurposeReporter plasmid for full-site selection assayDepositorInsertCRISPR_Cat
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD_CRISPR
Plasmid#120370PurposePlasmid containing shortened version of V-D CRISPR array for integration assaysDepositorInsertVD_CRISPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.sgKras.9
Plasmid#91894PurposesgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.DepositorInsertKras sgRNA
UseLentiviralTagsExpressionMutationPromoterU6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_RAF1_94
Plasmid#111826PurposeKnockout Raf1DepositorInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
J-SPRTNC-1
Plasmid#166521PurposescFv of a human scaffold targeting SprT-like N-terminal domain. Antigen coverage aa 1-216 of 489DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+1D
Plasmid#149389PurposeP. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation1bp inserted downstream of the IHFprox sitePromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPRmTmG2
Plasmid#69992PurposeThe CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.DepositorInsertgRNA that targets near LoxP sites
UseTagsExpressionMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg2
Plasmid#166999PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 2
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_RNeoStop_WPRE_bgh_pA
Plasmid#97011PurposeRosa26 targeting vector for Dre-responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
epiCRISPRe
Plasmid#135962PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_downstream_sgRNA
Plasmid#235610PurposeEncodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus downstream sgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_upstream_sgRNA
Plasmid#235609PurposeEncodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus upstream sgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228374PurposeExpresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLentiCRISPRv2_sgALDH7A1
Plasmid#218273PurposeCRISPR KO plasmid for ALDH7A1 in human cell linesDepositorInsertInserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targetting tdTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR22
Plasmid#217307PurposePromoterless (SwaI Cutsite) amdS:mRuby3 fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR23
Plasmid#217308PurposePromoterless (SwaI Cutsite) amdS:mNeonGreen fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR26
Plasmid#217311PurposeGAL7(p)-amdS:mRuby3 fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterGAL7Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR27
Plasmid#217312PurposeTEF1(p)-amdS:mRuby3 fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR28
Plasmid#217313PurposeGAL7(p)-amdS:mNeonGreen fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterGAL7Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR29
Plasmid#217314PurposeTEF1(p)-amdS:mNeonGreen fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only