Skip to main content
Addgene
Showing: 41 - 60 of 11894 results

  1. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

    Enlarge

  2. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    ER-SPLICSs-cFAST10
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210431-plasmid-map-sequence-id-415626

    Enlarge

  6. pFHMSP-LIC-C

    Plasmid
    #26100
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Sept. 15, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pLIC-SGC1

    Plasmid
    #39187
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39187-plasmid-map-sequence-id-160533

    Enlarge

  13. pAB-LIC C

    Plasmid
    #62288
    Purpose
    Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    8XHis tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. LIC-Z-YFP

    Plasmid
    #153544
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Venus YFP
    Expression
    Mammalian
    Mutation
    with mCherry in LIC-Z replaced by YFP
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153544-plasmid-map-sequence-id-305908

    Enlarge

  16. pBLIC-neo

    Plasmid
    #45198
    Purpose
    a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.
    Depositor
    Priyamvada Rai
    Article
    Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pBLIC-puro

    Plasmid
    #45197
    Purpose
    a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.
    Depositor
    Priyamvada Rai
    Article
    Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 19, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 60 of 11894 results