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Plasmid#203757PurposeEncodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorInsertNRas c-term (NRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shIFNAR
Plasmid#127699PurposeDoxycyclin inducible shRNA knockdown of mouse IFNAR geneDepositorInsertshIFNAR1 Mmu (Ifnar1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU
Plasmid#31726DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutation*PromoterNoneAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shSTING-Mmu
Plasmid#127697PurposeDoxycyclin inducible shRNA knockdown of mouse STING geneDepositorInsertshSTING Mmu (Sting1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA_Tet3
Plasmid#85740PurposeshRNA against mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseAAVTagsEYFPExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
UseShuttle vector for insect expression vectorsTagsExpressionMutationcodons altered for Drosophila and to eliminate re…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-TET1_CD
Plasmid#83570PurposeExpresses the catalytic domain of TET1 in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseTags3X FLAG tag and hr GFP IIExpressionMammalianMutationTET1 amino acids 1-1417 deletedPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBB HA RelA
Plasmid#74892PurposeExpress RelA in mammalian cellsDepositorInsertRelA (RELA Human)
UseTagsHAExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2
Plasmid#37845DepositorTypeEmpty backboneUseZebrafish enhancer assay vectorTagsExpressionMutationPromoterE1bAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5a-Myc
Plasmid#186613PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5A (KIF5A Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo1-NIK3x_pTRE-G418
Plasmid#202017Purposeexpresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vectorDepositorInsertV5-LOV-Turbo1-NIK3x
UseLentiviralTagsV5ExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST12.2 DYRK2 WT
Plasmid#20005DepositorInsertDYRK2 (DYRK2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
YF-Cdc42(CA)
Plasmid#20152DepositorInsertYFP-FKBP-Cdc42(CA) (CDC42 Human)
UseTagsExpressionMammalianMutationCdc42H was inserted into the multiple cloning sit…PromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
p3015b
Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
UseTagsExpressionBacterialMutationPromoterXyl/tet promoter that is constitutively activeAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-Kaede (Kaede-A69T)
Plasmid#99228PurposeBacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).DepositorInsertpr-Kaede
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD077
Plasmid#136473PurposeSSB-mTur2 IDL fusion expression plasmidDepositorInsertSSB-mTur2 (ssb E. coli)
UseTagsmTurquoise2ExpressionBacterialMutationmTurquoise2 inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
L2-AA007
Plasmid#185750PurposeVector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
UseTagsmembrane localization tag Lti6bExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway
Plasmid#37846DepositorTypeEmpty backboneUseZebrafish enhancer assay vectorTagsExpressionMutationPromoterE1bAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
Plasmid#79152PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloningDepositorInsertLshC2c2 locus
UseTagsExpressionMutation*PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only