Addgene

Showing: 5961 - 5980 of 11784 results

Results per page:

Sort results by:

pTBL516 CT sgRNA

Plasmid

#126433

Purpose

To target a protospacer with CT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CT at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL515 AA sgRNA

Plasmid

#126426

Purpose

To target a protospacer with AA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AA at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL509 AT sgRNA

Plasmid

#126429

Purpose

To target a protospacer with AT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AT at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL511 CC sgRNA

Plasmid

#126431

Purpose

To target a protospacer with CC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CC at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL508 TA sgRNA

Plasmid

#126438

Purpose

To target a protospacer with TA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TA at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL250 TC sgRNA

Plasmid

#126439

Purpose

To target a protospacer with TC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TC at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL514 TG sgRNA

Plasmid

#126440

Purpose

To target a protospacer with TG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TG at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL513 TT sgRNA

Plasmid

#126441

Purpose

To target a protospacer with TT at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TT at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL322 GC

Plasmid

#126950

Purpose

To target a protospacer with GC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GC at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL581 GA

Plasmid

#126951

Purpose

To target a protospacer with GA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GA at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL601 AG

Plasmid

#126952

Purpose

To target a protospacer with AG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AG at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL602 CG

Plasmid

#126953

Purpose

To target a protospacer with CG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CG at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-myr-Citrine-SV40pA-FKF

Plasmid

#119865

Purpose

Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO-Fab_Theo-LUCID

Plasmid

#119048

Purpose

Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Theo-LUCID_part1
Fab_Theo-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO-Fab_Quin-LUCID

Plasmid

#119049

Purpose

Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Quin-LUCID_part1
Fab_Quin-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO-Fab_MTX-LUCID

Plasmid

#119047

Purpose

Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_MTX-LUCID_part1
Fab_MTX-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs210-major

Plasmid

#113377

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs210-minor

Plasmid

#113378

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs438-major

Plasmid

#113369

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only
rs344-minor

Plasmid

#113366

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 minor allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs34466261 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge