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Showing: 5961 - 5980 of 16015 results

  4. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72453-plasmid-map-sequence-id-133893

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  5. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  6. pJK403

    Plasmid
    #72416
    Purpose
    Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72416-plasmid-map-sequence-id-135537

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  7. pJK405

    Plasmid
    #72418
    Purpose
    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72418-plasmid-map-sequence-id-135782

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  8. pJK195

    Plasmid
    #73321
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioC
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains silent mutation in Val428
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73321-plasmid-map-sequence-id-135029

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  9. pJK266

    Plasmid
    #73326
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73326-plasmid-map-sequence-id-135034

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  10. OTUD5

    Plasmid
    #28270
    Depositor
    Cheryl Arrowsmith
    Insert
    OTUD5 (OTUD5 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    OTU DOMAIN (residues 172-339).
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. WARS_pNIC-CTB10H

    Plasmid
    #153058
    Purpose
    Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    WARS (WARS1 Human)
    Use
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153058-plasmid-map-sequence-id-304478

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  12. YARS_pNIC-CTB10H

    Plasmid
    #153059
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    YARS (YARS1 Human)
    Use
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153059-plasmid-map-sequence-id-304479

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  13. YARS2_pNIC-CTB10H

    Plasmid
    #153060
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase 2
    Depositor
    Susanne Gräslund
    Insert
    YARS2 (YARS2 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153060-plasmid-map-sequence-id-304480

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  14. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

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  15. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

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  16. pcDNA3.1-StrepII

    Plasmid
    #208617
    Purpose
    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Strep II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    208617-plasmid-map-sequence-id-418313

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  17. FLAG-WT-short-UBASH3A

    Plasmid
    #192090
    Purpose
    Mammalian expression of human wild-type short-form UBASH3A with FLAG tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    192090-plasmid-map-sequence-id-382972

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  18. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  19. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid
    #218190
    Purpose
    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Insert
    nucA
    Use
    Tags
    His and NSP4 signal secretion peptide
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    218190-plasmid-map-sequence-id-433866

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  20. CAGGS-safb∆sap-NLS-3XFLAG-V5

    Plasmid
    #196091
    Purpose
    Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 31-65 (SAP domain) of mouse S…
    Promoter
    CAGGS
    Availability
    Academic Institutions and Nonprofits only
    196091-plasmid-map-sequence-id-384675

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Showing: 5961 - 5980 of 16015 results