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Plasmid#185877PurposeA parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pminiTol2-HuC-NE2h
Plasmid#208689PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafishDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseCloning vectorTagsExpressionMutationPromoterHucAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP(1-10)
Plasmid#70219PurposeExpresses GFP(1-10) in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertGFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRepCap6
Plasmid#110770PurposepRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.DepositorInsertAAV6 cap, AAV6 rep genes
UseTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD10
Plasmid#31332DepositorInsertPSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro
Plasmid#8453Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-N1
Plasmid#54642PurposeLocalization: N1 Cloning Vector, Excitation: 554, Emission: 581DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRC0940 (circRNA entry vector)
Plasmid#189967PurposeCloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestionDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9TagsExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
LeGO-dKatushka2
Plasmid#85214PurposeLentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoterDepositorInsertdKatushka2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationBsrGI site removed at 5' end, BsrGI site ins…PromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJT194
Plasmid#202293PurposeType IIS cloning plasmid for LPattB5 integrations w/ OriTDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG536
Plasmid#105423PurposeSynthetic biologyDepositorInsertNuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (GFP)
Plasmid#102944PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)DepositorInsertSIINFEKL (OVAL Chicken, Synthetic)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG
Plasmid#175503PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG Luciferase
Plasmid#98391PurposeGateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tagDepositorInsertLuciferase
UseEntry vector for gateway cloningTags3xFLAGExpressionMutationPromoterTRE tight and TRE tight promoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEM047 [T7-assisted CROPseq sgRNA expression vector]
Plasmid#224899PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNADepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a
Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA1A
Plasmid#19510DepositorInsertheat shock 70kDa protein 1A (HSPA1A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGTMV
Plasmid#118755PurposeBinary plasmid with a tobacco mosaic virus infectious cloneDepositorInsertTobacco mosaic virus
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only