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pUF-LbCpf1-pre-gRNA

Plasmid

#137848

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG and gRNA cassette

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.dTomato

Plasmid

#89392

Purpose

Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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Nsp2-SmuCas9_AAV

Plasmid

#192144

Purpose

Expresses Nsp2-SmuCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

Nsp2-SmuCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAC155-pCR8-sgExpression

Plasmid

#49045

Purpose

U6-driven sgRNA expressing cassette on a gateway donor vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_mMSH2

Plasmid

#186156

Purpose

sgRNA targeting murine Msh2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

Msh2_gRNA (Msh2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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KA2601_eSpCas9-G2P

Plasmid

#124203

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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LentiU6-hNEUROD1 gRNA_1-MS2-Puro

Plasmid

#192676

Purpose

Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human NEUROD1 activating gRNA #1 (NEUROD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-HA

Plasmid

#99370

Purpose

Expresses Cas9-HA and sgRNA. Hygromycin resistance.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-HA

Expression

Mutation

Promoter

Ribi promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLG_GI4

Plasmid

#111595

Purpose

GI Library Vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174119

Purpose

Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6 and UbC

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTJV1Sc-rpoB

Plasmid

#166978

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

Ec86 Retron (ECD_00831 )
lambda bet

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101 and VanCC

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_TOP1

Plasmid

#68423

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFA0055

Plasmid

#131774

Purpose

Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Article

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer CASS5a guide (gCASS5a) and 5' sgRNA

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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KA2058_pX330A-G2P

Plasmid

#124202

Purpose

Expression of SpCas9 and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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LentiU6-mNeurog2 gRNA_1-MS2-Puro

Plasmid

#192680

Purpose

Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-35S

Plasmid

#99861

Purpose

Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Expression

Mutation

Promoter

Ribi promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

Plasmid

#192681

Purpose

Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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doraWT_rescue_construct

Plasmid

#190609

Purpose

Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_A2S-8

Plasmid

#188654

Purpose

AAV vector expressing SaAID-2S and gRNA

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S--nSaCas9-UGI
Sa-sgRNA

Use

AAV and CRISPR

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

Scp1 and U6

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_sgEPAS1#1

Plasmid

#183122

Purpose

pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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