Skip to main content
Addgene
Showing: 5921 - 5940 of 15722 results

  5. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid
    #223175
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    223175-plasmid-map-sequence-id-457165

    Enlarge

  8. pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

    Plasmid
    #197902
    Purpose
    For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.
    Depositor
    Bo Wang
    Article
    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:
    Insert
    HSALNG0104472-163 (HSALNG0104472-scramble-sequence)
    Use
    Lentiviral
    Tags
    zsGreen1
    Expression
    Mammalian
    Mutation
    15q11.2 deletion
    Promoter
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    197902-plasmid-map-sequence-id-389136

    Enlarge

  9. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

    Enlarge

  10. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-450823

    Enlarge

  11. Pdg459-eSp(1.1) V3

    Plasmid
    #226965
    Purpose
    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226965-plasmid-map-sequence-id-450820

    Enlarge

  12. DG SaCas9 puro V3

    Plasmid
    #226960
    Purpose
    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226960-plasmid-map-sequence-id-450810

    Enlarge

  13. pCW57-CMV-MAAP6

    Plasmid
    #218245
    Purpose
    1) Ectopic expression of MAAP6 protein in mammalian cells. 2) Packaging MAAP6 sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein from Adeno-Associated Virus 6 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218245-plasmid-map-sequence-id-449799

    Enlarge

  14. pCW57-CMV-MAAP-SL08V9

    Plasmid
    #218251
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218251-plasmid-map-sequence-id-449683

    Enlarge

  19. pCW57-CMV-MAAP-SL01

    Plasmid
    #218248
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218248-plasmid-map-sequence-id-449801

    Enlarge

  20. pCW57-CMV-MAAP9

    Plasmid
    #218246
    Purpose
    1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218246-plasmid-map-sequence-id-449800

    Enlarge

Showing: 5921 - 5940 of 15722 results