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L2E-2

Plasmid

#21194

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek2 (Esr1, MAP2K2 Human, Mouse)

Use

Retroviral

Tags

Expression

Mutation

deletion of residues EQQKKRLE, S222D S226D in Me…

Promoter

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
IRS1-B1

Plasmid

#25698

Depositor

Eric Fearon

Article

Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

Insert

IRS1 (IRS1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
mycBioID-pBabePuro-myc-BirA*NRASG12V

Plasmid

#122212

Purpose

Stably expresses myc-BirA* fused to NRASG12V in mammalian cells

Depositor

Christopher Counter

Article

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

NRASG12V (NRAS Human)

Use

Retroviral

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMflPT-o4

Plasmid

#101315

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
pac resistance gene

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgSHMT2_2

Plasmid

#106310

Purpose

Express Cas9 and sgRNA targeting SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-Kaptin

Plasmid

#100511

Purpose

Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

KPTN (KPTN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX61

Plasmid

#46852

Purpose

TOP Hupki-puro2A-Hupki-wt plasmid

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 wild type (TP53 Human, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-2

Plasmid

#109009

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #2 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE H-Ras 12V, 189L

Plasmid

#14718

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

H-ras (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V, 189L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

Plasmid

#43969

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Gal4/DBD

Expression

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.2

Plasmid

#78541

Purpose

Experimental plasmid

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.4

Plasmid

#78543

Purpose

Experimental plasmid

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and U6 (for expressing sgRNA)

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLenCMV-PMMars1CyAVPR2

Plasmid

#75226

Purpose

Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-AVPR2 (AVPR2 Synthetic)

Use

Lentiviral

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-puroGFP-Eps8WT

Plasmid

#74956

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pSicoR (Puro) NEMO shRNA2

Plasmid

#22506

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA2 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
IRS1-A4

Plasmid

#25697

Depositor

Eric Fearon

Article

Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

Insert

IRS1 (IRS1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLEX_970_puro_DEST_V5_LUC

Plasmid

#45290

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
mlp-hCHES1sh

Plasmid

#51845

Purpose

shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

shRNA against CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only