-
Plasmid#124898PurposeExpress Flag-Daxx in mamalian cellsDepositorInsertDeath domain-associated protein 6 (DAXX )
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG.CASP3-Myc
Plasmid#200119PurposeExpresses C-terminal Myc-tagged caspase 3 in mammalian cellsDepositorInsertcaspase 3 (CASP3 Human)
UseTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-FLK1 TM
Plasmid#65249PurposeExpression of dominant negative FLK1 in mammalian cellsDepositorInsertFLK1 TM (Kdr Mouse)
UseRetroviralTagsExpressionMammalianMutationdeleted AA 786-1346PromoterLTRAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha WT GFP
Plasmid#128819PurposeTo visualize Doc2alpha WT in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2-mCherry-iTrkA
Plasmid#106168Purposemammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)DepositorInsertCRY2PHR mCherry iTrkA (CRY2 Mustard Weed, Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha D291N mutant GFP
Plasmid#128821PurposeTo visualize Doc2alpha D291N mutant in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationD291NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta D303N mutant GFP
Plasmid#128822PurposeTo visualize Doc2beta D303N mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationD303NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
UTS2R-DuET
Plasmid#213386PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertUTS2R (UTS2R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc
Plasmid#200124PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc
Plasmid#200126PurposeRetroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mGM-CSF
Plasmid#74465Purposeexpression of mGM-CSF in mammalian cellsDepositorInsertgranulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Flag-Ube2v1
Plasmid#110069PurposeExpress Halo-Tev-Flag-Ube2v1 (human) in mammalian cellsDepositorInsertHalo-Tev-Flag-Ube2v1 (UBE2V1 Human)
UseTagsHalo-TEV-FlagExpressionMammalianMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHSG1C3
Plasmid#164423Purposesingle guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscALPSblasti-TMPRSS2 Blasti
Plasmid#158088PurposeExpresses TMPRSS2 in mammalian cellsDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-MmACE2 (Mouse)
Plasmid#158087PurposeExpresses mouse ACE2 in mammalian cellsDepositorInsertMouse ACE2 (Ace2 Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-Sox10
Plasmid#115242PurposeLentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)DepositorInsertSOX10 (SOX10 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-HsACE2 (human)
Plasmid#158081PurposeExpresses human ACE2 in mammalian cellsDepositorInsertHuman ACE2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-FcACE2 (Cat)
Plasmid#158082PurposeExpresses cat ACE2 in mammalian cellsDepositorInsertCat ACE2 (ACE2 )
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-SsACE2 (Pig)
Plasmid#158085PurposeExpresses pig ACE2 in mammalian cellsDepositorInsertPig ACE2 (ACE2 Sus scrofa)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only