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Showing: 5901 - 5920 of 6716 results

  2. pET15b I-SceI (Ctermhis) K223I

    Plasmid
    #195615
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C2694G, G2345C, G2351C, A3365T, A3366T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195615-plasmid-map-sequence-id-383680

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  3. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

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  6. KBB394

    Plasmid
    #185086
    Purpose
    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)
    Depositor
    Ken Belanger
    Article
    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4
    Insert
    NMA111
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Nma111 KKR 9-11 AAA; KRK 28-30 AAA
    Promoter
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    185086-plasmid-map-sequence-id-367451

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  7. OfCasp3a

    Plasmid
    #166053
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166053-plasmid-map-sequence-id-328272

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  8. PaCasp7a

    Plasmid
    #166051
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-7a
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166051-plasmid-map-sequence-id-328269

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  9. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  16. pET21a - MPX

    Plasmid
    #89619
    Purpose
    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    Mitocondrial Tryparedoxin peroxidase
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89619-plasmid-map-sequence-id-176691

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  17. pET21a - EtfA

    Plasmid
    #89616
    Purpose
    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    Electron transport flavoprotein alpha subunit
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89616-plasmid-map-sequence-id-176692

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  18. pET21a - EtfB

    Plasmid
    #89617
    Purpose
    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    electron-transfer-flavoprotein, alpha polypeptide
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89617-plasmid-map-sequence-id-176708

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Showing: 5901 - 5920 of 6716 results