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B*07:05:01

Plasmid

#203268

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*07:05:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*57:03:01

Plasmid

#203260

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*57:03:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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VN-PTEN

Plasmid

#89331

Purpose

expresses hPTEN fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Marciniak et al J Exp Med. 2017 Aug 7;214(8):2257-2269.

Insert

PTEN1 (PTEN Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-N-IDR

Plasmid

#166440

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-N-IDR(1-164) (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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A*68:01:01

Plasmid

#203292

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*68:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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sgAPC(B)

Plasmid

#85558

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pBABE-2xFLAG-2xSTREP-CCND2-Puro

Plasmid

#172628

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L308/311/325/318E (4LE)

Plasmid

#166466

Purpose

Expresses fusion of mEGFP and YAPS127A- L308/311/325/318E (4LE)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L308/311/325/318E (4LE) (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

YAPS127A- L308/311/325/318E (4LE)

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry

Plasmid

#172635

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ser

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-V12-neo

Plasmid

#156178

Purpose

Lentiviral vector for expression of RAP2A-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-V12-neo

Plasmid

#156175

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-neo

Plasmid

#156176

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-TEAD4-YBD

Plasmid

#166450

Purpose

Expresses fusion of mCherry and TEAD4-YAP binding domain

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-YBD(217-434) (TEAD4, YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-Dsup-deltaC

Plasmid

#90023

Purpose

To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Deletion of C-terminal region

Promoter

CMV

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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LV-AR-R599A-N600A

Plasmid

#171221

Purpose

Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR-R599A-N600A (AR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

AR-R599A-N600A

Promoter

EF-1a

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLL5.0 ShCavin1-EGFP

Plasmid

#187253

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

Insert

ShRNA for Cavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

Plasmid

#170196

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)

Use

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

Promoter

CMV51

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-WT-neo

Plasmid

#156177

Purpose

Lentiviral vector for expression of RAP2A-WT, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-N17-neo

Plasmid

#156179

Purpose

Lentiviral vector for expression of RAP2A-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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