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Addgene
Showing: 5901 - 5920 of 16015 results

  1. pJK616

    Plasmid
    #72460
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with an N-terminal His6 tag (H6TdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72460-plasmid-map-sequence-id-133770

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  2. sfCherry11-WT-PTPN22

    Plasmid
    #195384
    Purpose
    Mammalian expression of human wild-type PTPN22 with sfCherry(11) tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    sfCherry(11)
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195384-plasmid-map-sequence-id-386968

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  3. WT-short-UBASH3A-GFP11

    Plasmid
    #195385
    Purpose
    Mammalian expression of human wild-type short-form UBASH3A with GFP(11) tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    GFP(11)
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195385-plasmid-map-sequence-id-385211

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  4. pET28-iLOV-Rsn2

    Plasmid
    #61687
    Purpose
    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV
    Depositor
    Brian Smith
    Insert
    Ranaspumin-2
    Use
    Tags
    His and iLOV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    61687-plasmid-map-sequence-id-259998

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  5. pCMV-mNoc-3X FLAG

    Plasmid
    #90465
    Purpose
    expression of Mouse Nocturnin with 3x Flag tag
    Depositor
    Carla Green
    Insert
    Nocturnin (Noct Mouse)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    90465-plasmid-map-sequence-id-178050

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  6. pENTR CMVie-Lifeact-mCardinal L1-R5

    Plasmid
    #84392
    Purpose
    Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mCardinal
    Expression
    Mammalian
    Mutation
    Promoter
    CMV immediate early
    Availability
    Academic Institutions and Nonprofits only
    84392-plasmid-map-sequence-id-169693

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  7. pSLIK luciferase-V5 neo

    Plasmid
    #220364
    Purpose
    Lentiviral expression vector for an inducible V5-tagged luciferase construct
    Depositor
    Kevin Janes
    Insert
    luciferase
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    220364-plasmid-map-sequence-id-436883

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  8. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Kang Zhou
    Insert
    FAST-PETase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Availability
    Academic Institutions and Nonprofits only
    212152-plasmid-map-sequence-id-424774

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  9. FLAG-hMYOD1 (#794)

    Plasmid
    #78329
    Purpose
    Overexpression of human MYOD1 ORF with a N-terminal FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  10. FLAG-hPAX3 (#395)

    Plasmid
    #78335
    Purpose
    Overexpression of human PAX3 ORF with a N-terminal FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    PAX3 (PAX3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  11. pEGFP-C1-BBS1

    Plasmid
    #218711
    Purpose
    Expresses N-terminally EGFP-tagged BBS1 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    BBS1 (BBS1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    218711-plasmid-map-sequence-id-434142

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  12. pFA6a-SRM (sortase recognition motif)-kanMX6

    Plasmid
    #54489
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    kanMX6
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. SIRT6

    Plasmid
    #28271
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. HT-ELK1

    Plasmid
    #33258
    Depositor
    Frederick Stanley
    Insert
    ELK1 (ELK1 Human)
    Use
    Tags
    Halo Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  15. NARS_pNIC-Bio3

    Plasmid
    #153054
    Purpose
    Expression construct for producing Avi tagged Asparaginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    NARS (NARS Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153054-plasmid-map-sequence-id-304473

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  16. TARS_pNIC-Bio3

    Plasmid
    #153057
    Purpose
    Expression construct for producing Avi tagged Threonyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    TARS (TARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    153057-plasmid-map-sequence-id-304477

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  17. pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53205
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and kanMX6
    Expression
    Mutation
    Promoter
    Gal1 promoter
    Availability
    Academic Institutions and Nonprofits only

  18. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53206
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and TRP1
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Availability
    Academic Institutions and Nonprofits only

  19. pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53207
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and HIS3MX6
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Availability
    Academic Institutions and Nonprofits only

  20. pJ609 Flag-Sumo1

    Plasmid
    #49046
    Purpose
    Mammalian expression of N-terminally tagged Sumo1
    Depositor
    Lianna Swanson
    Insert
    SUMO1 (SUMO1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49046-plasmid-map-sequence-id-72029

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Showing: 5901 - 5920 of 16015 results