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expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .
expresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi
DNA assembly cloning (NEB).
Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.