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pDe_Cas9_KANA_R390a

Plasmid

#173758

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/390a_sgRNA2/390a

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU6 &StU6

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_316

Plasmid

#180547

Purpose

Entry vector containing sfGFP

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_344

Plasmid

#180548

Purpose

Entry vector containing cjBlue

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

cjBlue

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

Plasmid

#186418

Purpose

Expression of scrambled shRNA used as a control for knock down experiments

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

ScramblemiR

Use

AAV

Tags

mCitrine

Expression

Mutation

Promoter

human Synapsin1

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

Plasmid

#186419

Purpose

Expression of shRNA for the knock down of mouse Egr3

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

miREgr3-5D

Use

AAV

Tags

mCitrine

Expression

Mutation

Promoter

human Synapsin1

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Mouse-caspase-1/11a

Plasmid

#183391

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pNB101

Plasmid

#169110

Purpose

Feedback-controlled AraC circuit

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

araC (araC Escherichia coli K12 MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB200

Plasmid

#169111

Purpose

TESEC backbone for target expression

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

lacZ (lacZ Escherichia coli K12 MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB201

Plasmid

#169112

Purpose

TESEC expression vector for Alr from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

alr (alr Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB203

Plasmid

#169114

Purpose

TESEC expression vector for mEGFP-Alr fusion construct

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB204

Plasmid

#169115

Purpose

TESEC expression vector for 6xHis-Alr fusion constrcut

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

6xHis-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB205

Plasmid

#169116

Purpose

TESEC expression vector for Asd from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

asd (asd Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB206

Plasmid

#169117

Purpose

TESEC expression vector for CysH from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

cysH (cysH Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB207

Plasmid

#169118

Purpose

TESEC expression vector for DapB from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

dapB (dapB Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB208

Plasmid

#169119

Purpose

TESEC expression vector for TrpD from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

trpD (trpD Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb21

Plasmid

#184679

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb21 (S Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_345

Plasmid

#180550

Purpose

Entry vector containing eforRed

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

eforRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Mouse-caspase-1/11b

Plasmid

#183392

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of mouse caspase-1 fused to mouse caspase-11 (Casp1 Synthetic, Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_009

Plasmid

#180518

Purpose

Empty backbone for D1.1 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_137

Plasmid

#180521

Purpose

Empty backbone for D1.2 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge